Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3633258..3634057 Replicon chromosome
Accession NZ_CP076527
Organism Escherichia coli strain L3452210II

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KPE21_RS17600 Protein ID WP_000347251.1
Coordinates 3633258..3633722 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KPE21_RS17605 Protein ID WP_001307405.1
Coordinates 3633722..3634057 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE21_RS17570 (3628259) 3628259..3628693 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KPE21_RS17575 (3628711) 3628711..3629589 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KPE21_RS17580 (3629579) 3629579..3630358 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KPE21_RS17585 (3630369) 3630369..3630842 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KPE21_RS17590 (3630865) 3630865..3632145 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KPE21_RS17595 (3632394) 3632394..3633203 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KPE21_RS17600 (3633258) 3633258..3633722 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KPE21_RS17605 (3633722) 3633722..3634057 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KPE21_RS17610 (3634206) 3634206..3635777 - 1572 WP_001273761.1 galactarate dehydratase -
KPE21_RS17615 (3636152) 3636152..3637486 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
KPE21_RS17620 (3637502) 3637502..3638272 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T205927 WP_000347251.1 NZ_CP076527:c3633722-3633258 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T205927 NZ_CP101925:c4232528-4232214 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205927 WP_001307405.1 NZ_CP076527:c3634057-3633722 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205927 NZ_CP101925:c4232764-4232531 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References