Detailed information of TA system TA205909

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33034..33303
• Replicon: plasmid pK186_2
• Accession: NZ_CP076520
• Organism: Klebsiella pneumoniae strain K186

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KNW01_RS27645
• Protein ID: WP_001372321.1
• Coordinates: 33178..33303 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	33034..33099 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNW01_RS27620	28744..29271	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
KNW01_RS27625	29329..29562	+	234	WP_000006003.1	DUF905 family protein	-
KNW01_RS27630	29623..31646	+	2024	Protein_41	ParB/RepB/Spo0J family partition protein	-
KNW01_RS27635	31715..32149	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KNW01_RS27640	32146..32865	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	32877..33101	+	225	NuclAT_0	-	-
-	32877..33101	+	225	NuclAT_0	-	-
-	32877..33101	+	225	NuclAT_0	-	-
-	32877..33101	+	225	NuclAT_0	-	-
-	33034..33099	-	66	-	-	Antitoxin
KNW01_RS28300	33087..33236	+	150	Protein_44	plasmid maintenance protein Mok	-
KNW01_RS27645	33178..33303	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KNW01_RS27650	33622..33918	-	297	Protein_46	hypothetical protein	-
KNW01_RS27655	34218..34514	+	297	WP_001272251.1	hypothetical protein	-
KNW01_RS27660	34625..35446	+	822	WP_001234445.1	DUF932 domain-containing protein	-
KNW01_RS27665	35743..36333	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
KNW01_RS27670	36668..37051	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KNW01_RS27675	37245..37916	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
KNW01_RS27680	38053..38280	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB	-	1..78128	78128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T205909 WP_001372321.1 NZ_CP076520:33178-33303 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205909 NZ_CP101923:37096-37617 [Escherichia coli]
GTGGATAATATAAAGATAGAAATTTTCTCTGGAGAGAAAGATTATGATTTGAATGGTTTTGATTGCGGTGAAGAATCACT
AAATGCCTTTTTAACCAACCACTTAAAAAGGCAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACGAAAGAAG
GCAGACCAAAAGTGTTAGGGTATTACACTTTGTCAGGTAGCTGCTTTGAAAAGGAGTCCTTACCTTCAAGAAGCCAGCAA
AAGAAGGTACCTTATCGGAATGTTCCAAGTGTTACTTTGGGTAGGCTGGCTTTGGACAAGTCCCTTCAAGGTCAAGGATT
TGGCTCAATGCTTGTAACACATGCAATGCGCGTTGTGTACAATGCATCTCTTGCTGTAGGTATTCATGGACTTTTCGTTG
AGGCGTTAAATGACAAAGCCAAGGCGTTTTATAAAAGTTTAGACTTTATCCAGCTGGTTGGTAACAACGAGCGTTCTTTG
TTCTATCCTACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT205909 NZ_CP076520:c33099-33034 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin