Detailed information of TA system TA205874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 17282..17618
• Replicon: plasmid pUAMSEL1
• Accession: NZ_CP076499
• Organism: Enterococcus faecalis strain UAMS_EL53

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: KPU58_RS16500
• Protein ID: WP_141435800.1
• Coordinates: 17472..17618 (-)
• Length: 49 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	17282..17538 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU58_RS14985	13007..13495	+	489	WP_002398545.1	hypothetical protein	-
KPU58_RS14990	13913..14095	+	183	WP_001808764.1	hypothetical protein	-
KPU58_RS14995	14102..14368	+	267	WP_001245899.1	hypothetical protein	-
KPU58_RS15000	14530..15306	+	777	WP_002398548.1	Cas9 inhibitor AcrIIA9 family protein	-
KPU58_RS15005	15303..16637	+	1335	WP_002398549.1	PcfJ domain-containing protein	-
KPU58_RS15010	16777..17253	+	477	WP_196008545.1	single-stranded DNA-binding protein	-
-	17282..17538	+	257	-	-	Antitoxin
KPU58_RS16500	17472..17618	-	147	WP_141435800.1	putative holin-like toxin	Toxin
KPU58_RS15015	18190..18387	+	198	WP_001031355.1	hypothetical protein	-
KPU58_RS15020	18528..19463	+	936	WP_002367766.1	hypothetical protein	-
KPU58_RS15025	19537..19680	+	144	WP_000066155.1	hypothetical protein	-
KPU58_RS15030	19677..20120	+	444	WP_001209505.1	hypothetical protein	-
KPU58_RS15035	20110..20973	+	864	WP_000865436.1	DNA/RNA non-specific endonuclease	-
KPU58_RS16470	20976..21107	+	132	WP_000159353.1	hypothetical protein	-
KPU58_RS15040	21097..22410	+	1314	WP_002398555.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III	cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI	1..119720	119720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-48)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5382.49 Da
• Isoelectric Point: 10.6867

>T205874 WP_141435800.1 NZ_CP076499:c17618-17472 [Enterococcus faecalis]
MNVLPKSIERRSLLSVSESLQLMLAFGGFTLTLITTIVAILNYKDKKK

Nucleotide

• Length: 147 bp

>T205874 NZ_CP101921:1003453-1003788 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 257 bp

>AT205874 NZ_CP076499:17282-17538 [Enterococcus faecalis]
CACCGCCACCGCCGTTTAGGCGCGCATGAAAAAAGGTAGGCATTCGCCTACCTTTTCGCACAAAATGTGGTATAATAATT
ATGTAAAGAGGACGTACTGGTAATACGCCCTCATTGATAGCACCGCTAAAGACGGTGGCAGAGTTTTTATAAGTTATTTA
GAAATAACCGGTCTCCTGCTAAAGTGACGGTTATTTCTTTTTGTCTTTATAGTTCAGAATGGCAACAATGGTTGTGATTA
GGGTCAATGTAAACCCA

Structures

Antitoxin