Detailed information of TA system TA205835

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 105236..105572
• Replicon: plasmid pUAMSEL1
• Accession: NZ_CP076494
• Organism: Enterococcus faecalis strain UAMS_EL54

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: KPU55_RS16505
• Protein ID: WP_141435800.1
• Coordinates: 105236..105382 (+)
• Length: 49 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	105316..105572 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU55_RS15570	100445..101758	-	1314	WP_002398555.1	hypothetical protein	-
KPU55_RS16480	101748..101879	-	132	WP_000159353.1	hypothetical protein	-
KPU55_RS15575	101882..102745	-	864	WP_000865436.1	DNA/RNA non-specific endonuclease	-
KPU55_RS15580	102735..103178	-	444	WP_001209505.1	hypothetical protein	-
KPU55_RS15585	103175..103318	-	144	WP_000066155.1	hypothetical protein	-
KPU55_RS15590	103392..104327	-	936	WP_002367766.1	hypothetical protein	-
KPU55_RS15595	104468..104665	-	198	WP_001031355.1	hypothetical protein	-
KPU55_RS16505	105236..105382	+	147	WP_141435800.1	putative holin-like toxin	Toxin
-	105316..105572	-	257	-	-	Antitoxin
KPU55_RS15605	105601..106077	-	477	WP_196008545.1	single-stranded DNA-binding protein	-
KPU55_RS15610	106217..107551	-	1335	WP_002398549.1	PcfJ domain-containing protein	-
KPU55_RS15615	107548..108324	-	777	WP_002398548.1	Cas9 inhibitor AcrIIA9 family protein	-
KPU55_RS15620	108486..108752	-	267	WP_001245899.1	hypothetical protein	-
KPU55_RS15625	108759..108941	-	183	WP_001808764.1	hypothetical protein	-
KPU55_RS15630	109360..109848	-	489	WP_002398545.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	cylI / cylA / cylB / cylM / cylS / cylL / cylR1 / cylR2	1..119714	119714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-48)

Sequences

Toxin

Protein

• Length: 49 a.a.
• Molecular weight: 5382.49 Da
• Isoelectric Point: 10.6867

>T205835 WP_141435800.1 NZ_CP076494:105236-105382 [Enterococcus faecalis]
MNVLPKSIERRSLLSVSESLQLMLAFGGFTLTLITTIVAILNYKDKKK

Nucleotide

• Length: 147 bp

>T205835 NZ_CP101915:2649224-2649331 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 257 bp

>AT205835 NZ_CP076494:c105572-105316 [Enterococcus faecalis]
CACCGCCACCGCCGTTTAGGCGCGCATGAAAAAAGGTAGGCATTCGCCTACCTTTTCGCACAAAATGTGGTATAATAATT
ATGTAAAGAGGACGTACTGGTAATACGCCCTCATTGATAGCACCGCTAAAGACGGTGGCAGAGTTTTTATAAGTTATTTA
GAAATAACCGGTCTCCTGCTAAAGTGACGGTTATTTCTTTTTGTCTTTATAGTTCAGAATGGCAACAATGGTTGTGATTA
GGGTCAATGTAAACCCA

Structures

Antitoxin