Detailed information of TA system TA020583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2466516..2467033
• Replicon: chromosome
• Accession: NC_002951
• Organism: Staphylococcus aureus subsp. aureus COL

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: SACOL_RS12615
• Protein ID: WP_000113262.1
• Coordinates: 2466516..2466782 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: SACOL_RS12620
• Protein ID: WP_000584499.1
• Coordinates: 2466782..2467033 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACOL_RS12590	2461942..2462478	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
SACOL_RS12595	2462711..2463535	-	825	WP_000572040.1	formate/nitrite transporter family protein	-
SACOL_RS12600	2463752..2463934	-	183	WP_000230294.1	hypothetical protein	-
SACOL_RS12605	2464018..2464485	-	468	WP_000153344.1	SRPBCC domain-containing protein	-
SACOL_RS12610	2464671..2466221	-	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SACOL_RS15150	2466410..2466481	-	72	Protein_2381	hypothetical protein	-
SACOL_RS12615	2466516..2466782	-	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
SACOL_RS12620	2466782..2467033	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SACOL_RS12625	2467198..2467299	-	102	WP_001791744.1	hypothetical protein	-
SACOL_RS12630	2467306..2467905	-	600	WP_000162813.1	DsbA family protein	-
SACOL_RS12635	2467924..2468286	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SACOL_RS12640	2468541..2469791	+	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
SACOL_RS12645	2469888..2470619	-	732	WP_000615497.1	amino acid ABC transporter ATP-binding protein	-
SACOL_RS12650	2470616..2471335	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T20583 WP_000113262.1 NC_002951:c2466782-2466516 [Staphylococcus aureus subsp. aureus COL]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T20583 NC_002951:c2466782-2466516 [Staphylococcus aureus subsp. aureus COL]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT20583 WP_000584499.1 NC_002951:c2467033-2466782 [Staphylococcus aureus subsp. aureus COL]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT20583 NC_002951:c2467033-2466782 [Staphylococcus aureus subsp. aureus COL]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60