Detailed information of TA system TA205812

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2314244..2314476
• Replicon: chromosome
• Accession: NZ_CP076493
• Organism: Enterococcus faecalis strain UAMS_EL54

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q837V5
• Locus tag: KPU55_RS11250
• Protein ID: WP_002366178.1
• Coordinates: 2314360..2314476 (-)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2314244..2314449 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU55_RS11230	2309731..2310819	-	1089	WP_002355572.1	diacylglycerol kinase	-
KPU55_RS11235	2310838..2312268	-	1431	WP_002358703.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
KPU55_RS11240	2312268..2313737	-	1470	WP_002361259.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
KPU55_RS11245	2313737..2314042	-	306	WP_002355569.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
-	2314244..2314449	+	206	-	-	Antitoxin
KPU55_RS11250	2314360..2314476	-	117	WP_002366178.1	putative holin-like toxin	Toxin
KPU55_RS11255	2314624..2316654	-	2031	WP_002361258.1	NAD-dependent DNA ligase LigA	-
KPU55_RS11260	2316780..2319038	-	2259	WP_002363179.1	DNA helicase PcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-35)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4108.01 Da
• Isoelectric Point: 5.9482

>T205812 WP_002366178.1 NZ_CP076493:c2314476-2314360 [Enterococcus faecalis]
MFLSVEAALGLMIGFATLVVTIIFVILALVLDNKNNRS

Nucleotide

• Length: 117 bp

>T205812 NZ_CP101912:c149006-148725 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Nucleotide

• Length: 206 bp

>AT205812 NZ_CP076493:2314244-2314449 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAA
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A1G1S9M0

Antitoxin