Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 197977..198512 Replicon plasmid pUAMSEF1
Accession NZ_CP076484
Organism Enterococcus faecium strain UAMS_EF55

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag KPU59_RS14785 Protein ID WP_002305108.1
Coordinates 197977..198249 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag KPU59_RS14790 Protein ID WP_002303154.1
Coordinates 198249..198512 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU59_RS14755 (KPU59_14720) 192983..194170 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
KPU59_RS14760 (KPU59_14725) 194358..195305 + 948 WP_002303155.1 sugar-binding domain-containing protein -
KPU59_RS14765 (KPU59_14730) 195602..196150 - 549 WP_002292681.1 hypothetical protein -
KPU59_RS14770 (KPU59_14735) 196151..197008 - 858 WP_002292680.1 ParA family protein -
KPU59_RS14775 (KPU59_14740) 197294..197581 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
KPU59_RS14780 (KPU59_14745) 197571..197900 - 330 WP_002292678.1 AbrB family transcriptional regulator -
KPU59_RS14785 (KPU59_14750) 197977..198249 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
KPU59_RS14790 (KPU59_14755) 198249..198512 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPU59_RS14795 (KPU59_14760) 198618..198941 - 324 WP_224453712.1 hypothetical protein -
KPU59_RS14800 (KPU59_14765) 199040..199603 - 564 Protein_214 IS6-like element ISS1N family transposase -
KPU59_RS14805 (KPU59_14770) 199683..200845 + 1163 WP_086956687.1 IS3 family transposase -
KPU59_RS14810 (KPU59_14775) 201317..202216 - 900 WP_002303235.1 aldose 1-epimerase family protein -
KPU59_RS14815 (KPU59_14780) 202237..202509 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..264192 264192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T205760 WP_002305108.1 NZ_CP076484:c198249-197977 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T205760 NZ_CP101877:1904918-1905020 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT205760 WP_002303154.1 NZ_CP076484:c198512-198249 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT205760 NZ_CP101877:c1905027-1904882 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References