Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 139221..139756 Replicon plasmid pUAMSEF1
Accession NZ_CP076478
Organism Enterococcus faecium strain UAMS_EF57

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag KPU56_RS14470 Protein ID WP_002305108.1
Coordinates 139221..139493 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag KPU56_RS14475 Protein ID WP_002303154.1
Coordinates 139493..139756 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPU56_RS14440 (KPU56_14400) 134227..135414 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
KPU56_RS14445 (KPU56_14405) 135602..136549 + 948 WP_002303155.1 sugar-binding domain-containing protein -
KPU56_RS14450 (KPU56_14410) 136846..137394 - 549 WP_002292681.1 hypothetical protein -
KPU56_RS14455 (KPU56_14415) 137395..138252 - 858 WP_002292680.1 ParA family protein -
KPU56_RS14460 (KPU56_14420) 138538..138825 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
KPU56_RS14465 (KPU56_14425) 138815..139144 - 330 WP_002292678.1 AbrB family transcriptional regulator -
KPU56_RS14470 (KPU56_14430) 139221..139493 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
KPU56_RS14475 (KPU56_14435) 139493..139756 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KPU56_RS14480 (KPU56_14440) 139862..140185 - 324 WP_224453712.1 hypothetical protein -
KPU56_RS14485 (KPU56_14445) 140284..140847 - 564 Protein_149 IS6-like element ISS1N family transposase -
KPU56_RS14490 (KPU56_14450) 140927..142089 + 1163 WP_086956687.1 IS3 family transposase -
KPU56_RS14495 (KPU56_14455) 142561..143460 - 900 WP_002303235.1 aldose 1-epimerase family protein -
KPU56_RS14500 (KPU56_14460) 143481..143753 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..205436 205436


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T205755 WP_002305108.1 NZ_CP076478:c139493-139221 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T205755 NZ_CP101867:105709-105858 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT205755 WP_002303154.1 NZ_CP076478:c139756-139493 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT205755 NZ_CP101867:c105665-105604 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References