Detailed information of TA system TA205751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 139192..139727
• Replicon: plasmid pUAMSEF1
• Accession: NZ_CP076472
• Organism: Enterococcus faecium strain UAMS_EF58

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q3Y221
• Locus tag: KPU57_RS14460
• Protein ID: WP_002305108.1
• Coordinates: 139192..139464 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A133CED7
• Locus tag: KPU57_RS14465
• Protein ID: WP_002303154.1
• Coordinates: 139464..139727 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPU57_RS14430 (KPU57_14400)	134198..135385	-	1188	WP_002303156.1	NADP-dependent malic enzyme	-
KPU57_RS14435 (KPU57_14405)	135573..136520	+	948	WP_002303155.1	sugar-binding domain-containing protein	-
KPU57_RS14440 (KPU57_14410)	136817..137365	-	549	WP_002292681.1	hypothetical protein	-
KPU57_RS14445 (KPU57_14415)	137366..138223	-	858	WP_002292680.1	ParA family protein	-
KPU57_RS14450 (KPU57_14420)	138509..138796	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KPU57_RS14455 (KPU57_14425)	138786..139115	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
KPU57_RS14460 (KPU57_14430)	139192..139464	-	273	WP_002305108.1	Txe/YoeB family addiction module toxin	Toxin
KPU57_RS14465 (KPU57_14435)	139464..139727	-	264	WP_002303154.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KPU57_RS14470 (KPU57_14440)	139833..140156	-	324	WP_224453712.1	hypothetical protein	-
KPU57_RS14475 (KPU57_14445)	140255..140818	-	564	Protein_149	IS6-like element ISS1N family transposase	-
KPU57_RS14480 (KPU57_14450)	140898..142060	+	1163	WP_086956687.1	IS3 family transposase	-
KPU57_RS14485 (KPU57_14455)	142532..143431	-	900	WP_002303235.1	aldose 1-epimerase family protein	-
KPU57_RS14490 (KPU57_14460)	143452..143724	-	273	WP_002305107.1	DUF3884 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..205407	205407

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10806.28 Da
• Isoelectric Point: 9.9080

>T205751 WP_002305108.1 NZ_CP076472:c139464-139192 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T205751 NZ_CP101866:c3911109-3910822 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9886.16 Da
• Isoelectric Point: 4.5370

>AT205751 WP_002303154.1 NZ_CP076472:c139727-139464 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT205751 NZ_CP101866:c3910835-3910533 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A133CER3

Antitoxin

• AlphaFold DB: A0A133CED7