Detailed information of TA system TA020574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): TMCS/-
• Location: 359972..360551
• Replicon: chromosome
• Accession: NC_002951
• Organism: Staphylococcus aureus subsp. aureus COL

Toxin (Protein)

• Gene name: MW1434
• Uniprot ID: A0A4P7P609
• Locus tag: SACOL_RS01645
• Protein ID: WP_001025401.1
• Coordinates: 359972..360217 (+)
• Length: 82 a.a.

Antitoxin (RNA)

Gene name	MW1433
Locus tag	-
Coordinates	360186..360551 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SACOL_RS01610	356184..356897	-	714	WP_001582614.1	type II toxin-antitoxin system PemK/MazF family toxin	-
SACOL_RS01615	356975..357157	-	183	WP_000705240.1	hypothetical protein	-
SACOL_RS01620	357357..358076	-	720	WP_000358220.1	XRE family transcriptional regulator	-
SACOL_RS01625	358218..358436	+	219	WP_001198674.1	helix-turn-helix domain-containing protein	-
SACOL_RS01630	358451..358759	+	309	WP_001153965.1	hypothetical protein	-
SACOL_RS01635	358916..359707	+	792	WP_001148570.1	phage antirepressor KilAC domain-containing protein	-
SACOL_RS01640	359708..359932	+	225	WP_000187184.1	hypothetical protein	-
SACOL_RS01645	359972..360217	+	246	WP_001025401.1	DUF2829 domain-containing protein	Toxin
SACOL_RS01650	360186..360551	-	366	WP_001128433.1	hypothetical protein	Antitoxin
SACOL_RS01655	360608..360805	+	198	WP_031903992.1	hypothetical protein	-
SACOL_RS01660	360792..361172	-	381	WP_000773060.1	DUF2513 domain-containing protein	-
SACOL_RS01665	361220..361411	+	192	WP_000389907.1	hypothetical protein	-
SACOL_RS01670	361389..361640	-	252	WP_000801109.1	hypothetical protein	-
SACOL_RS01675	361695..361958	+	264	WP_001124196.1	helix-turn-helix domain-containing protein	-
SACOL_RS01680	361971..362132	+	162	WP_001285962.1	DUF1270 domain-containing protein	-
SACOL_RS01685	362222..362539	+	318	WP_001097093.1	hypothetical protein	-
SACOL_RS01690	362544..362804	+	261	WP_001556704.1	DUF1108 family protein	-
SACOL_RS01695	362814..363035	+	222	WP_000815401.1	DUF2483 family protein	-
SACOL_RS01700	363028..363651	+	624	WP_000139722.1	DUF1071 domain-containing protein	-
SACOL_RS01705	363651..364070	+	420	WP_000934777.1	single-stranded DNA-binding protein	-
SACOL_RS01710	364084..364779	+	696	WP_001124444.1	hypothetical protein	-
SACOL_RS01715	364751..365551	+	801	WP_000504976.1	phage replisome organizer N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	354785..397516	42731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF2829 (2-69)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9468.72 Da
• Isoelectric Point: 5.7783

>T20574 WP_001025401.1 NC_002951:359972-360217 [Staphylococcus aureus subsp. aureus COL]
MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISNSDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQ
F

Nucleotide

• Length: 246 bp

>T20574 NC_002951:359972-360217 [Staphylococcus aureus subsp. aureus COL]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Nucleotide

• Length: 366 bp

>AT20574 NC_002951:c360551-360186 [Staphylococcus aureus subsp. aureus COL]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A4P7P609

Antitoxin