Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 2582732..2583425 Replicon chromosome
Accession NZ_CP076470
Organism Escherichia coli strain DH5a

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag KN807_RS12495 Protein ID WP_000415584.1
Coordinates 2582732..2583028 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag KN807_RS12500 Protein ID WP_000650107.1
Coordinates 2583030..2583425 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KN807_RS12460 (2577820) 2577820..2578134 - 315 WP_000958598.1 putative quinol monooxygenase -
KN807_RS12465 (2578165) 2578165..2578746 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
KN807_RS12470 (2579065) 2579065..2579397 + 333 WP_000917684.1 DUF2645 family protein -
KN807_RS12475 (2579443) 2579443..2580792 - 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
KN807_RS12480 (2580789) 2580789..2581448 - 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB -
KN807_RS12485 (2581600) 2581600..2581992 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
KN807_RS12490 (2582045) 2582045..2582527 + 483 WP_000183505.1 GyrI-like domain-containing protein -
KN807_RS12495 (2582732) 2582732..2583028 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
KN807_RS12500 (2583030) 2583030..2583425 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
KN807_RS12505 (2583558) 2583558..2585165 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
KN807_RS12510 (2585303) 2585303..2587561 + 2259 WP_001281881.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T205731 WP_000415584.1 NZ_CP076470:2582732-2583028 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T205731 NZ_CP101860:c79280-78999 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT205731 WP_000650107.1 NZ_CP076470:2583030-2583425 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT205731 NZ_CP101860:c79521-79270 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References