Detailed information of TA system TA020572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4750051..4750751
• Replicon: chromosome
• Accession: NC_002947
• Organism: Pseudomonas putida KT2440

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q88F93
• Locus tag: PP_RS21835
• Protein ID: WP_010954960.1
• Coordinates: 4750455..4750751 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: I7AUJ7
• Locus tag: PP_RS21830
• Protein ID: WP_003254235.1
• Coordinates: 4750051..4750452 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP_RS21810 (PP_4200)	4745185..4746006	-	822	WP_010954957.1	transporter substrate-binding domain-containing protein	-
PP_RS21815 (PP_4201)	4746082..4747011	-	930	WP_010954958.1	FAD-binding protein	-
PP_RS21820 (PP_4202)	4747012..4747761	-	750	WP_003254232.1	electron transfer flavoprotein subunit beta/FixA family protein	-
PP_RS21825 (PP_4203)	4748297..4749979	+	1683	WP_010954959.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
PP_RS21830 (PP_4204)	4750051..4750452	-	402	WP_003254235.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
PP_RS21835 (PP_4205)	4750455..4750751	-	297	WP_010954960.1	type II toxin-antitoxin system MqsR family toxin	Toxin
PP_RS21840 (PP_4206)	4750846..4752171	-	1326	WP_010954961.1	MFS transporter	-
PP_RS21845 (PP_4207)	4752244..4752723	-	480	WP_010954962.1	hypothetical protein	-
PP_RS21850 (PP_4208)	4752892..4753368	-	477	WP_010954963.1	sigma-70 family RNA polymerase sigma factor	-
PP_RS21855 (PP_4209)	4753581..4754759	+	1179	WP_010954964.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (6-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-109)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11133.22 Da
• Isoelectric Point: 10.2271

>T20572 WP_010954960.1 NC_002947:c4750751-4750455 [Pseudomonas putida KT2440]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Nucleotide

• Length: 297 bp

>T20572 NC_002947:c4750751-4750455 [Pseudomonas putida KT2440]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14684.76 Da
• Isoelectric Point: 4.9531

>AT20572 WP_003254235.1 NC_002947:c4750452-4750051 [Pseudomonas putida KT2440]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT20572 NC_002947:c4750452-4750051 [Pseudomonas putida KT2440]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATTCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Toxin

• AlphaFold DB: Q88F93

Antitoxin

• AlphaFold DB: I7AUJ7