Detailed information of TA system TA205696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 358222..358868
• Replicon: chromosome
• Accession: NZ_CP076453
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: KOM10_RS01655
• Protein ID: WP_016529833.1
• Coordinates: 358222..358569 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W9BB18
• Locus tag: KOM10_RS01660
• Protein ID: WP_016529832.1
• Coordinates: 358569..358868 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS01645 (KOM10_01635)	354148..355581	+	1434	WP_002920564.1	glycogen synthase GlgA	-
KOM10_RS01650 (KOM10_01640)	355599..358046	+	2448	WP_002920561.1	glycogen phosphorylase	-
KOM10_RS01655 (KOM10_01645)	358222..358569	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KOM10_RS01660 (KOM10_01650)	358569..358868	+	300	WP_016529832.1	helix-turn-helix transcriptional regulator	Antitoxin
KOM10_RS01665 (KOM10_01655)	358931..360439	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
KOM10_RS01670 (KOM10_01660)	360644..360973	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
KOM10_RS01675 (KOM10_01665)	361024..361854	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
KOM10_RS01680 (KOM10_01670)	361904..362662	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T205696 WP_016529833.1 NZ_CP076453:358222-358569 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T205696 NZ_CP101844:866845-867102 [Streptococcus suis]
TTGAGTTATAAGGTTATTTTGTCTGCTAAGGCCCAAAAGCAAATTCGAAAGATGGATAAAACCGCTGCAAGTTTGATTTT
GCGCTATCTCTACAAGCATATTGACGGTTGCGACAACCCTAGACAATACGGAAAAGCCTTGACTGCTAATCGTTCTGGCC
AATGGCGCTATCGTATCGGCAATTACAGGGTTATCGTTAATATTGAAGATGATAAATTGATTGTGACAAGCATAAAAATG
AGAAATGGAAGTATATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.63 Da
• Isoelectric Point: 10.4041

>AT205696 WP_016529832.1 NZ_CP076453:358569-358868 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNAPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT205696 NZ_CP101844:866616-866855 [Streptococcus suis]
ATGGCAGCTATTACATTGAAAGTTTCTGAGCAGGACAAGCTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTCAGTTT
GTCAGAACTCATTCGGACTAAAACCCTTGAAGCCCTTGAAGACGAGTACGATGCGCGTGTGGCAGACGTTGCTTGGGCAG
AATACCAAGCAGACTTGGCGAATAGAATTGAGCCGATGACATTAGAACAAATGGCTGAGGAGTTGGGCATTGAGTTATAA

Structures

Toxin

• AlphaFold DB: W9BBY1

Antitoxin

• AlphaFold DB: W9BB18