Detailed information of TA system TA205692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): tisB-istR/TisB(toxin)
• Location: 32310..32685
• Replicon: chromosome
• Accession: NZ_CP076453
• Organism: Klebsiella pneumoniae strain PM27

Toxin (Protein)

• Gene name: tisB
• Uniprot ID: A0A0H3GV52
• Locus tag: KOM10_RS00160
• Protein ID: WP_004145059.1
• Coordinates: 32310..32429 (-)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	istR
Locus tag	-
Coordinates	32606..32685 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOM10_RS00135 (KOM10_00135)	28324..28428	+	105	WP_040146642.1	hypothetical protein	-
KOM10_RS00140 (KOM10_00140)	28428..29351	+	924	WP_004150274.1	DNA-binding transcriptional regulator DsdC	-
KOM10_RS00145 (KOM10_00145)	29511..30695	-	1185	WP_004181587.1	multidrug efflux MFS transporter EmrD	-
KOM10_RS00150 (KOM10_00150)	30871..31704	-	834	WP_002923296.1	DMT family transporter	-
KOM10_RS00155 (KOM10_00155)	31773..32219	-	447	WP_002923294.1	GNAT family N-acetyltransferase	-
KOM10_RS00160 (KOM10_00160)	32310..32429	-	120	WP_004145059.1	type I toxin-antitoxin system toxin TisB	Toxin
-	32606..32685	+	80	-	-	Antitoxin
KOM10_RS00165 (KOM10_00165)	32633..32800	-	168	WP_014343455.1	hypothetical protein	-
KOM10_RS00170 (KOM10_00170)	32813..32908	+	96	WP_002923286.1	ilvB operon leader peptide IvbL	-
KOM10_RS00175 (KOM10_00175)	33013..34701	+	1689	WP_004173858.1	acetolactate synthase large subunit	-
KOM10_RS00180 (KOM10_00180)	34705..34992	+	288	WP_002923283.1	acetolactate synthase small subunit	-
KOM10_RS00185 (KOM10_00185)	35143..35736	+	594	WP_002923282.1	transcriptional regulator UhpA	-
KOM10_RS00190 (KOM10_00190)	35754..37235	+	1482	WP_004151519.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin TisB, type I toxin-antitoxin system (11-38)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4292.34 Da
• Isoelectric Point: 7.9965

>T205692 WP_004145059.1 NZ_CP076453:c32429-32310 [Klebsiella pneumoniae]
MCSLTTGDARMGGMDIIILILKLMVAVLQLLDAVLKQFR

Nucleotide

• Length: 120 bp

>T205692 NZ_CP101835:c5268100-5267717 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 80 bp

>AT205692 NZ_CP076453:32606-32685 [Klebsiella pneumoniae]
TGTTGACGTAACACAGTGTGCTTGCGGCTACCACCTGTAACCATGCTGATAAAAACCTCGCTCCGGCGGGGTTTTTTGTT

Structures

Toxin

• AlphaFold DB: A0A0H3GV52

Antitoxin