Detailed information of TA system TA205684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1702885..1703531
• Replicon: chromosome
• Accession: NZ_CP076449
• Organism: Aggregatibacter actinomycetemcomitans strain CU1000N

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KO461_RS08415
• Protein ID: WP_005565157.1
• Coordinates: 1702885..1703235 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: X2JT85
• Locus tag: KO461_RS08420
• Protein ID: WP_005540827.1
• Coordinates: 1703232..1703531 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KO461_RS08395 (KO461_08400)	1698070..1698315	-	246	WP_005540815.1	YgjV family protein	-
KO461_RS08400 (KO461_08405)	1698506..1699843	-	1338	WP_005540817.1	argininosuccinate synthase	-
KO461_RS08405 (KO461_08410)	1699931..1700758	-	828	WP_005540818.1	hypothetical protein	-
KO461_RS08410 (KO461_08415)	1701115..1702698	+	1584	WP_053330249.1	peptide chain release factor 3	-
KO461_RS08415 (KO461_08420)	1702885..1703235	+	351	WP_005565157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KO461_RS08420 (KO461_08425)	1703232..1703531	+	300	WP_005540827.1	helix-turn-helix transcriptional regulator	Antitoxin
KO461_RS08425 (KO461_08430)	1703567..1706500	-	2934	WP_005565158.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.69 Da
• Isoelectric Point: 9.9664

>T205684 WP_005565157.1 NZ_CP076449:1702885-1703235 [Aggregatibacter actinomycetemcomitans]
MWVVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T205684 NZ_CP101835:771769-772254 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11342.12 Da
• Isoelectric Point: 5.8598

>AT205684 WP_005540827.1 NZ_CP076449:1703232-1703531 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATLKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT205684 NZ_CP101835:771479-771772 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: X2JT85