20567

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1777558..1778144 Replicon chromosome
Accession NC_002947
Organism Pseudomonas putida KT2440

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag PP_RS08185 Protein ID WP_010952670.1
Coordinates 1777866..1778144 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag PP_RS08180 Protein ID WP_010952669.1
Coordinates 1777558..1777857 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PP_RS08155 (PP_1581) 1772818..1774809 + 1992 WP_010952665.1 glycoside hydrolase -
PP_RS08160 (PP_1582) 1774821..1775897 + 1077 WP_010952666.1 hypothetical protein -
PP_RS08165 (PP_1583) 1775958..1776401 + 444 WP_049587424.1 structural protein P5 -
PP_RS08170 (PP_1584) 1776398..1776898 + 501 WP_010952668.1 DUF2514 domain-containing protein -
PP_RS08175 (PP_5476) 1776964..1777266 + 303 WP_049587425.1 hypothetical protein -
PP_RS08180 (PP_1585) 1777558..1777857 - 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
PP_RS08185 (PP_1586) 1777866..1778144 - 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
PP_RS08190 (PP_1587) 1778395..1780041 + 1647 WP_049587427.1 Na+/H+ antiporter -
PP_RS08195 (PP_1588) 1780147..1781343 - 1197 WP_010952672.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T20567 WP_010952670.1 NC_002947:c1778144-1777866 [Pseudomonas putida KT2440]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T20567 NC_002947:c1778144-1777866 [Pseudomonas putida KT2440]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGCGGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGACCAGCACAGTATCCGTGTAAATGACCAATGGCGGTTGTGCTTCACCTGGACAGAG
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT20567 WP_010952669.1 NC_002947:c1777857-1777558 [Pseudomonas putida KT2440]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT20567 NC_002947:c1777857-1777558 [Pseudomonas putida KT2440]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACCCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References