20566

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1449214..1450004 Replicon chromosome
Accession NC_002947
Organism Pseudomonas putida KT2440

Toxin (Protein)


Gene name yhaV Uniprot ID Q88NE5
Locus tag PP_RS06565 Protein ID WP_010952397.1
Coordinates 1449214..1449681 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID I7C311
Locus tag PP_RS06570 Protein ID WP_014859917.1
Coordinates 1449678..1450004 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PP_RS06545 (PP_1263) 1444377..1445906 + 1530 WP_010952395.1 efflux transporter outer membrane subunit -
PP_RS06550 (PP_1264) 1445903..1448080 + 2178 WP_003251716.1 FUSC family protein -
PP_RS06555 (PP_1265) 1448070..1448270 + 201 WP_003251718.1 DUF1656 domain-containing protein -
PP_RS06560 (PP_1266) 1448281..1449141 + 861 WP_010952396.1 HlyD family secretion protein -
PP_RS06565 (PP_1267) 1449214..1449681 - 468 WP_010952397.1 type II toxin-antitoxin system YhaV family toxin Toxin
PP_RS06570 (PP_1268) 1449678..1450004 - 327 WP_014859917.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
PP_RS06575 (PP_1269) 1450097..1450543 - 447 WP_010952399.1 universal stress protein -
PP_RS06580 (PP_1270) 1450817..1451722 + 906 WP_010952400.1 LysR family transcriptional regulator -
PP_RS06585 (PP_1271) 1451839..1453380 + 1542 WP_010952401.1 MFS transporter -
PP_RS06590 (PP_1272) 1453409..1454470 + 1062 WP_049587435.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(17-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17962.34 Da        Isoelectric Point: 9.7963

>T20566 WP_010952397.1 NC_002947:c1449681-1449214 [Pseudomonas putida KT2440]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Download         Length: 468 bp

>T20566 NC_002947:c1449681-1449214 [Pseudomonas putida KT2440]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTAATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAAAAGAAATACCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGATCAATGACGAGTCGAGCAAGCGGGCCTATGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATAGCGGCAACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 11919.40 Da        Isoelectric Point: 4.3722

>AT20566 WP_014859917.1 NC_002947:c1450004-1449678 [Pseudomonas putida KT2440]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp

>AT20566 NC_002947:c1450004-1449678 [Pseudomonas putida KT2440]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88NE5

Antitoxin

Source ID Structure
AlphaFold DB I7C311

References