20565

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1373331..1373932 Replicon chromosome
Accession NC_002947
Organism Pseudomonas putida KT2440

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag PP_RS06205 Protein ID WP_049587984.1
Coordinates 1373618..1373932 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag PP_RS06200 Protein ID WP_049587986.1
Coordinates 1373331..1373618 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PP_RS06180 (PP_1194) 1368363..1369208 + 846 WP_003254799.1 hypothetical protein -
PP_RS06185 (PP_1195) 1369205..1370923 + 1719 WP_010952348.1 hypothetical protein -
PP_RS06190 (PP_1196) 1370978..1371727 + 750 WP_049587988.1 hypothetical protein -
PP_RS06195 (PP_1197) 1371799..1373130 - 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
PP_RS06200 (PP_1198) 1373331..1373618 - 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
PP_RS06205 (PP_1199) 1373618..1373932 - 315 WP_049587984.1 hypothetical protein Toxin
PP_RS06210 (PP_1200) 1374381..1376291 + 1911 WP_010952353.1 potassium transporter Kup -
PP_RS06215 (PP_1201) 1376340..1377632 - 1293 WP_010952354.1 virulence factor family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T20565 WP_049587984.1 NC_002947:c1373932-1373618 [Pseudomonas putida KT2440]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

>T20565 NC_002947:c1373932-1373618 [Pseudomonas putida KT2440]
ATGATCTTCATCGAGACCCCAGTATTCACCTCCGATCTCAAGGAGCACCTGGATGATGAAGAATACCGGGCTTTGCAGGC
CTATCTGGCCGAGCATCCCGAAGCCGGTTCTCTCCTGGAGGAAACCGGCGGCCTCAGAAAGATCCGCTGGGCAGCCAAGG
GCAAGGGCAAGAGTGGGGGTGTACGAGTGATCTACTACCACGTCACTGCAGCCCACCAGATCCGTATGATTCTGATCTAT
CGCAAGGGCATCGTGGATACCTTGACAAGCAGTCAGAAAGCGCAGCTTCGCGCACTAAACAAAGGGTGGAAATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT20565 WP_049587986.1 NC_002947:c1373618-1373331 [Pseudomonas putida KT2440]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp

>AT20565 NC_002947:c1373618-1373331 [Pseudomonas putida KT2440]
ATGGAAAAGCATCTCTTCGATCGTCTTGTGGAAAGCATGACTCAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCCTC
GCGTGAGTTCCACGTAGACGCACTTCAAGTCAAAAAGATTCGTCAAGCCACTGGCCTTTCCCAGGCTGCGTTTGCCAAAC
GTATCGATGTCGCAGTAGGAACTCTACGGAATTGGGAACAGGGCCGTCGCGAACCTGAAGGACCAGCTCGTGCCTTGCTT
CGAGCGATTCATAACGATCCCGAACATGTACTTGCGGCCTTGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References