Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3180289..3181016 Replicon chromosome
Accession NZ_CP076404
Organism Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KJY95_RS15420 Protein ID WP_000550189.1
Coordinates 3180702..3181016 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KJY95_RS15415 Protein ID WP_000560266.1
Coordinates 3180289..3180705 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJY95_RS15405 (3175449) 3175449..3177800 + 2352 WP_000695487.1 alpha-glucosidase -
KJY95_RS15410 (3178226) 3178226..3180244 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KJY95_RS15415 (3180289) 3180289..3180705 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KJY95_RS15420 (3180702) 3180702..3181016 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KJY95_RS15425 (3181300) 3181300..3182436 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KJY95_RS15430 (3182521) 3182521..3183024 + 504 WP_001333820.1 M48 family metallopeptidase -
KJY95_RS15435 (3183101) 3183101..3183793 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KJY95_RS15440 (3183872) 3183872..3184858 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T205645 WP_000550189.1 NZ_CP076404:c3181016-3180702 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T205645 NZ_CP101784:2014287-2014389 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT205645 WP_000560266.1 NZ_CP076404:c3180705-3180289 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT205645 NZ_CP101784:c2014396-2014251 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References