Detailed information of TA system TA205644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3114413..3115106
• Replicon: chromosome
• Accession: NZ_CP076404
• Organism: Escherichia coli strain K-12 substr. AB1157

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KJY95_RS15110
• Protein ID: WP_000415584.1
• Coordinates: 3114810..3115106 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: KJY95_RS15105
• Protein ID: WP_000650107.1
• Coordinates: 3114413..3114808 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJY95_RS15095 (3110277)	3110277..3112535	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
KJY95_RS15100 (3112673)	3112673..3114280	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
KJY95_RS15105 (3114413)	3114413..3114808	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
KJY95_RS15110 (3114810)	3114810..3115106	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KJY95_RS15115 (3115311)	3115311..3115793	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
KJY95_RS15120 (3115846)	3115846..3116238	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KJY95_RS15125 (3116390)	3116390..3117049	+	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
KJY95_RS15130 (3117046)	3117046..3118395	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
KJY95_RS15135 (3118441)	3118441..3118773	-	333	WP_000917684.1	DUF2645 family protein	-
KJY95_RS15140 (3119092)	3119092..3119673	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KJY95_RS15145 (3119704)	3119704..3120018	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T205644 WP_000415584.1 NZ_CP076404:c3115106-3114810 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T205644 NZ_CP101784:882453-882860 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT205644 WP_000650107.1 NZ_CP076404:c3114808-3114413 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT205644 NZ_CP101784:882206-882472 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65