Detailed information of TA system TA205599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 998244..998938
• Replicon: chromosome
• Accession: NZ_CP076386
• Organism: Dickeya dadantii strain S3-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: KNV89_RS04230
• Protein ID: WP_280528194.1
• Coordinates: 998244..998648 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: KNV89_RS04235
• Protein ID: WP_027711146.1
• Coordinates: 998645..998938 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNV89_RS04230 (KNV89_04230)	998244..998648	-	405	WP_280528194.1	type II toxin-antitoxin system YafO family toxin	Toxin
KNV89_RS04235 (KNV89_04235)	998645..998938	-	294	WP_027711146.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KNV89_RS04240 (KNV89_04240)	999339..1000775	+	1437	WP_216283638.1	TIR domain-containing protein	-
KNV89_RS04245 (KNV89_04245)	1001198..1002631	-	1434	WP_216283639.1	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
KNV89_RS04250 (KNV89_04250)	1002979..1003263	-	285	WP_216283640.1	conjugal transfer protein TraD	-
KNV89_RS04255 (KNV89_04255)	1003260..1003514	-	255	WP_039275709.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (29-132)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15511.91 Da
• Isoelectric Point: 7.5156

>T205599 WP_280528194.1 NZ_CP076386:c998648-998244 [Dickeya dadantii]
MSIRIFKSALIRQQLSQQELDDLAADFLSYKKDGVLPDIFGRDAPYDDDRTYPLVKEEQVAHIHLADADAPFPKFLRQFK
RTSDQAHLVYCQGAMEPNAYLLIIILKPEAHKMARNNNHMHKIGMMAEAFRMKH

Nucleotide

• Length: 405 bp

>T205599 NZ_CP101765:c272218-271775 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10979.44 Da
• Isoelectric Point: 4.9167

>AT205599 WP_027711146.1 NZ_CP076386:c998938-998645 [Dickeya dadantii]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEEKQGRVHTAARFRPSAERLSDIADSG
QKLLQNASDKDLTEFTE

Nucleotide

• Length: 294 bp

>AT205599 NZ_CP101765:c272445-272215 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA