Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2825375..2825877 Replicon chromosome
Accession NZ_CP076344
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag KM937_RS13915 Protein ID WP_000767822.1
Coordinates 2825375..2825629 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KM937_RS13920 Protein ID WP_001259255.1
Coordinates 2825626..2825877 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS13895 (2821868) 2821868..2823226 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
KM937_RS13900 (2823216) 2823216..2823278 - 63 WP_010723108.1 membrane protein YoeI -
KM937_RS13905 (2823493) 2823493..2824422 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
KM937_RS13910 (2824468) 2824468..2825292 - 825 WP_000754737.1 SDR family oxidoreductase -
KM937_RS13915 (2825375) 2825375..2825629 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KM937_RS13920 (2825626) 2825626..2825877 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KM937_RS13925 (2826160) 2826160..2826210 + 51 WP_001364200.1 his operon leader peptide -
KM937_RS13930 (2826356) 2826356..2827255 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
KM937_RS13935 (2827261) 2827261..2828565 + 1305 WP_001445483.1 histidinol dehydrogenase -
KM937_RS13940 (2828562) 2828562..2829632 + 1071 WP_001538918.1 histidinol-phosphate transaminase -
KM937_RS13945 (2829632) 2829632..2830699 + 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T205534 WP_000767822.1 NZ_CP076344:c2825629-2825375 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T205534 NZ_CP101719:1759932-1760219 [Xanthomonas oryzae pv. oryzae]
GTGGGTGGGGCCGGTCATGAGCAGCGTGGGGACCGGCCACACTTGGTTCTCAGTTCGCAAGGCTACAACGACACAACTAG
TCTAGTGGTTTGCGTTCCTGTCACAACTAAACCCAAAGGCAATCCTTTTGAAGTAAGCCTTAATGATTTGAACAAGCCAA
GTGTTGCTTTAACAAATCAGGTCATGACGGCAGATTGGCGCAGTAGAAATGCTTTTCCCCGAGGGTTTGCTAATAAAGCT
GAAATGCTCCAAGTTGAAGCCAAGGTTCGCGCTTTGCTTCAACTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT205534 WP_001259255.1 NZ_CP076344:c2825877-2825626 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT205534 NZ_CP101719:1759642-1759884 [Xanthomonas oryzae pv. oryzae]
ATGGCTGTTTTGAAGATGTGGGGGAATAGTGCTGCAGTTCGAATCCCGGCGACCTATCTCGAGGAGTCCAAGTTTTCTAT
TGACGAGCCTCTTGTAATCACTGCGGAGGAAGGTCGCATTGTGATCGAGTCGAGTAAGCCGTCTTATAACCTCGCGGACT
TGGTTGCTCAAATCACTCCAGAAAACACGCACGGCTTTGCTGGGCATTCCCAGAGAGTCGGGGCGGAGGATATTGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References