Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2273009..2273571 Replicon chromosome
Accession NZ_CP076344
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag KM937_RS11105 Protein ID WP_000605675.1
Coordinates 2273293..2273571 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KM937_RS11100 Protein ID WP_000781370.1
Coordinates 2273009..2273293 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS11085 (2268190) 2268190..2271237 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
KM937_RS11090 (2271250) 2271250..2272134 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
KM937_RS11095 (2272127) 2272127..2272780 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
KM937_RS11100 (2273009) 2273009..2273293 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KM937_RS11105 (2273293) 2273293..2273571 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KM937_RS11110 (2273757) 2273757..2274767 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
KM937_RS11115 (2274901) 2274901..2276598 - 1698 WP_000433460.1 malate dehydrogenase -
KM937_RS11120 (2276755) 2276755..2276892 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KM937_RS11125 (2276994) 2276994..2277209 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KM937_RS11130 (2277554) 2277554..2277985 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T205525 WP_000605675.1 NZ_CP076344:c2273571-2273293 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T205525 NZ_CP101705:2762876-2762983 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGTGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT205525 WP_000781370.1 NZ_CP076344:c2273293-2273009 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT205525 NZ_CP101705:c2762823-2762762 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References