Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1230653..1231332 Replicon chromosome
Accession NZ_CP076344
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag KM937_RS05840 Protein ID WP_000057523.1
Coordinates 1230653..1230955 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag KM937_RS05845 Protein ID WP_000806442.1
Coordinates 1230991..1231332 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS05820 (1225916) 1225916..1227136 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
KM937_RS05825 (1227354) 1227354..1229006 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
KM937_RS05830 (1229043) 1229043..1229522 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
KM937_RS05835 (1229726) 1229726..1230520 - 795 WP_001538398.1 TraB/GumN family protein -
KM937_RS05840 (1230653) 1230653..1230955 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KM937_RS05845 (1230991) 1230991..1231332 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
KM937_RS05850 (1231390) 1231390..1233894 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
KM937_RS05855 (1234156) 1234156..1235088 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T205524 WP_000057523.1 NZ_CP076344:1230653-1230955 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T205524 NZ_CP101705:2762340-2762447 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT205524 WP_000806442.1 NZ_CP076344:1230991-1231332 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT205524 NZ_CP101705:c2762293-2762227 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References