Detailed information of TA system TA205522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1002898..1003592
• Replicon: chromosome
• Accession: NZ_CP076344
• Organism: Escherichia coli strain AVS0096

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: KM937_RS04805
• Protein ID: WP_001263500.1
• Coordinates: 1003194..1003592 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KM937_RS04800
• Protein ID: WP_000554758.1
• Coordinates: 1002898..1003191 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM937_RS04775 (998715)	998715..999182	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
KM937_RS04780 (999202)	999202..999918	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
KM937_RS04785 (999931)	999931..1000794	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
KM937_RS04790 (1000797)	1000797..1001720	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
KM937_RS04795 (1001791)	1001791..1002846	+	1056	WP_001226168.1	DNA polymerase IV	-
KM937_RS04800 (1002898)	1002898..1003191	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KM937_RS04805 (1003194)	1003194..1003592	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KM937_RS04810 (1003602)	1003602..1004054	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
KM937_RS04815 (1004244)	1004244..1005383	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
KM937_RS04820 (1005380)	1005380..1005994	+	615	WP_000602124.1	peptide chain release factor H	-
KM937_RS04825 (1006051)	1006051..1007508	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
KM937_RS04830 (1007769)	1007769..1008227	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T205522 WP_001263500.1 NZ_CP076344:1003194-1003592 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T205522 NZ_CP101705:2136012-2136115 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT205522 WP_000554758.1 NZ_CP076344:1002898-1003191 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT205522 NZ_CP101705:c2136153-2135882 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3