Detailed information of TA system TA205515

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 611697..612109
• Replicon: chromosome
• Accession: NZ_CP076344
• Organism: Escherichia coli strain AVS0096

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: KM937_RS02935
• Protein ID: WP_000132614.1
• Coordinates: 611697..612038 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	612033..612109 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM937_RS02915 (606766)	606766..608046	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
KM937_RS02920 (608231)	608231..609151	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
KM937_RS02925 (609379)	609379..609459	+	81	WP_020233658.1	hypothetical protein	-
KM937_RS24730 (609557)	609557..610433	+	877	Protein_572	DUF262 domain-containing protein	-
KM937_RS24735 (610487)	610487..611650	+	1164	WP_224153787.1	DUF1524 domain-containing protein	-
KM937_RS02935 (611697)	611697..612038	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (612033)	612033..612109	+	77	NuclAT_8	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_8	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_8	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_8	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_9	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_9	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_9	-	Antitoxin
- (612033)	612033..612109	+	77	NuclAT_9	-	Antitoxin
KM937_RS02940 (612259)	612259..614028	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
KM937_RS02945 (614028)	614028..615497	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T205515 WP_000132614.1 NZ_CP076344:c612038-611697 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T205515 NZ_CP101705:979288-979695 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT205515 NZ_CP076344:612033-612109 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin