Detailed information of TA system TA205510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4322460..4323154
• Replicon: chromosome
• Accession: NZ_CP076318
• Organism: Escherichia coli strain Survcare045

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: KNN08_RS22350
• Protein ID: WP_001263489.1
• Coordinates: 4322756..4323154 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: KNN08_RS22345
• Protein ID: WP_000554758.1
• Coordinates: 4322460..4322753 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN08_RS22325 (4318092)	4318092..4318589	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
KNN08_RS22330 (4318813)	4318813..4320525	-	1713	Protein_4064	flagellar biosynthesis protein FlhA	-
KNN08_RS22335 (4320497)	4320497..4321282	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
KNN08_RS22340 (4321353)	4321353..4322408	+	1056	WP_001226164.1	DNA polymerase IV	-
KNN08_RS22345 (4322460)	4322460..4322753	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KNN08_RS22350 (4322756)	4322756..4323154	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KNN08_RS22355 (4323164)	4323164..4323616	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
KNN08_RS22360 (4323934)	4323934..4324140	+	207	Protein_4070	RtcB family protein	-
KNN08_RS22365 (4324136)	4324136..4324657	+	522	Protein_4071	peptide chain release factor H	-
KNN08_RS22370 (4324714)	4324714..4326171	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
KNN08_RS22375 (4326432)	4326432..4326890	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4327486)	4327486..4327566	+	81	NuclAT_12	-	-
- (4327486)	4327486..4327566	+	81	NuclAT_12	-	-
- (4327486)	4327486..4327566	+	81	NuclAT_12	-	-
- (4327486)	4327486..4327566	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T205510 WP_001263489.1 NZ_CP076318:4322756-4323154 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T205510 NZ_CP101705:325093-325323 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT205510 WP_000554758.1 NZ_CP076318:4322460-4322753 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT205510 NZ_CP101705:325320-325679 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3