Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4316416..4316957 Replicon chromosome
Accession NZ_CP076318
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag KNN08_RS22310 Protein ID WP_000615983.1
Coordinates 4316416..4316694 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag KNN08_RS22315 Protein ID WP_000729703.1
Coordinates 4316697..4316957 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS22295 (4313998) 4313998..4314576 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KNN08_RS22300 (4314782) 4314782..4315549 + 768 WP_000333380.1 class II glutamine amidotransferase -
KNN08_RS22305 (4315520) 4315520..4316260 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KNN08_RS22310 (4316416) 4316416..4316694 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KNN08_RS22315 (4316697) 4316697..4316957 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KNN08_RS22320 (4317167) 4317167..4317916 + 750 WP_000056849.1 C40 family peptidase -
KNN08_RS22325 (4318092) 4318092..4318589 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KNN08_RS22330 (4318813) 4318813..4320525 - 1713 Protein_4064 flagellar biosynthesis protein FlhA -
KNN08_RS22335 (4320497) 4320497..4321282 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T205509 WP_000615983.1 NZ_CP076318:c4316694-4316416 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205509 NZ_CP101704:c4862519-4862367 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT205509 WP_000729703.1 NZ_CP076318:c4316957-4316697 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205509 NZ_CP101704:c4862303-4862169 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References