Detailed information of TA system TA205482

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85636..86057
• Replicon: plasmid pS045-CMY-42
• Accession: NZ_CP076315
• Organism: Escherichia coli strain Survcare045

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KNN08_RS01150
• Protein ID: WP_096937776.1
• Coordinates: 85636..85761 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85859..86057 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN08_RS01115 (80700)	80700..80915	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
KNN08_RS01120 (81051)	81051..81698	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
KNN08_RS01125 (81889)	81889..82272	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KNN08_RS01130 (82642)	82642..83214	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
KNN08_RS01135 (83510)	83510..84331	-	822	WP_001234465.1	DUF932 domain-containing protein	-
KNN08_RS01140 (84450)	84450..84737	-	288	WP_000107242.1	hypothetical protein	-
KNN08_RS01145 (84762)	84762..84968	-	207	WP_000275854.1	hypothetical protein	-
KNN08_RS25135 (85038)	85038..85335	+	298	Protein_98	hypothetical protein	-
KNN08_RS01150 (85636)	85636..85761	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KNN08_RS25140 (85703)	85703..85852	-	150	Protein_100	DUF5431 family protein	-
- (85859)	85859..86057	-	199	NuclAT_0	-	Antitoxin
- (85859)	85859..86057	-	199	NuclAT_0	-	Antitoxin
- (85859)	85859..86057	-	199	NuclAT_0	-	Antitoxin
- (85859)	85859..86057	-	199	NuclAT_0	-	Antitoxin
KNN08_RS01155 (86026)	86026..86131	-	106	Protein_101	plasmid SOS inhibition protein A	-
KNN08_RS01160 (86286)	86286..86603	+	318	Protein_102	IS110 family transposase	-
KNN08_RS01165 (86892)	86892..87143	-	252	Protein_103	iron-containing alcohol dehydrogenase	-
KNN08_RS01170 (87228)	87228..87740	-	513	WP_223350513.1	potassium channel family protein	-
KNN08_RS01175 (87867)	87867..88560	-	694	Protein_105	IS1 family transposase	-
KNN08_RS01180 (88617)	88617..88721	+	105	Protein_106	thiosulfate reductase cytochrome B subunit	-
KNN08_RS01185 (88967)	88967..90361	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..167285	167285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T205482 WP_096937776.1 NZ_CP076315:c85761-85636 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205482 NZ_CP101689:2948502-2948993 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT205482 NZ_CP076315:c86057-85859 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin