Detailed information of TA system TA205477

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44382..44636
• Replicon: plasmid pS045-CMY-42
• Accession: NZ_CP076315
• Organism: Escherichia coli strain Survcare045

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: KNN08_RS00915
• Protein ID: WP_001367749.1
• Coordinates: 44382..44531 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44575..44636 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN08_RS00885 (40405)	40405..40806	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KNN08_RS00890 (40739)	40739..40996	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KNN08_RS00895 (41089)	41089..41742	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KNN08_RS00900 (42681)	42681..43538	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
KNN08_RS00905 (43531)	43531..43605	-	75	WP_001365705.1	RepA leader peptide Tap	-
KNN08_RS00910 (43841)	43841..44098	-	258	WP_000083833.1	replication regulatory protein RepA	-
KNN08_RS00915 (44382)	44382..44531	-	150	WP_001367749.1	Hok/Gef family protein	Toxin
- (44575)	44575..44636	+	62	NuclAT_1	-	Antitoxin
- (44575)	44575..44636	+	62	NuclAT_1	-	Antitoxin
- (44575)	44575..44636	+	62	NuclAT_1	-	Antitoxin
- (44575)	44575..44636	+	62	NuclAT_1	-	Antitoxin
KNN08_RS25125 (44775)	44775..45203	-	429	WP_029702151.1	hypothetical protein	-
KNN08_RS00925 (45416)	45416..45985	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
KNN08_RS00930 (46137)	46137..46763	-	627	WP_029702149.1	NYN domain-containing protein	-
KNN08_RS00935 (47175)	47175..47384	-	210	WP_039023209.1	hypothetical protein	-
KNN08_RS00940 (47515)	47515..48075	-	561	WP_001567328.1	fertility inhibition protein FinO	-
KNN08_RS00945 (48130)	48130..48876	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..167285	167285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T205477 WP_001367749.1 NZ_CP076315:c44531-44382 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205477 NZ_CP101689:c2035960-2035685 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGGGACAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT205477 NZ_CP076315:44575-44636 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin