Detailed information of TA system TA205471

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21191..21455
• Replicon: plasmid pS253-CMY-42
• Accession: NZ_CP076309
• Organism: Escherichia coli strain Survcare253

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: KNN07_RS24805
• Protein ID: WP_001387489.1
• Coordinates: 21191..21343 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	21395..21455 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN07_RS24770 (16283)	16283..16816	-	534	WP_001221666.1	lipocalin family protein	-
KNN07_RS24775 (16910)	16910..18055	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
KNN07_RS25165 (19260)	19260..19358	+	99	Protein_19	ethanolamine utilization protein EutE	-
KNN07_RS24785 (19430)	19430..19639	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KNN07_RS24790 (20031)	20031..20207	+	177	WP_001054897.1	hypothetical protein	-
KNN07_RS25275 (20272)	20272..20367	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
KNN07_RS24800 (20868)	20868..21119	+	252	WP_001291964.1	hypothetical protein	-
KNN07_RS24805 (21191)	21191..21343	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (21395)	21395..21455	+	61	NuclAT_0	-	Antitoxin
- (21395)	21395..21455	+	61	NuclAT_0	-	Antitoxin
- (21395)	21395..21455	+	61	NuclAT_0	-	Antitoxin
- (21395)	21395..21455	+	61	NuclAT_0	-	Antitoxin
KNN07_RS24810 (21664)	21664..22038	+	375	WP_223349261.1	hypothetical protein	-
KNN07_RS24815 (22191)	22191..23348	+	1158	Protein_26	IS1380-like element ISEcp1 family transposase	-
KNN07_RS24820 (23404)	23404..24101	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KNN07_RS24825 (24112)	24112..24420	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
KNN07_RS24830 (24862)	24862..25149	-	288	WP_000074855.1	conjugal transfer protein TraA	-
KNN07_RS25170 (25187)	25187..25345	-	159	Protein_30	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCMY-42	-	1..26256	26256
	-	inside	IScluster/Tn	blaCMY-42	-	16910..23679	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T205471 WP_001387489.1 NZ_CP076309:c21343-21191 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T205471 NZ_CP101667:c95010-94594 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT205471 NZ_CP076309:21395-21455 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin