Detailed information of TA system TA205470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 26958..27669
• Replicon: plasmid unnamed2
• Accession: NZ_CP076307
• Organism: Escherichia coli strain Survcare253

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: KNN07_RS24010
• Protein ID: WP_000162415.1
• Coordinates: 27367..27669 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KNN07_RS24005
• Protein ID: WP_000806445.1
• Coordinates: 26958..27296 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN07_RS23980 (KNN07_23980)	22240..23073	-	834	WP_226149389.1	hypothetical protein	-
KNN07_RS23985 (KNN07_23985)	23420..24073	+	654	WP_023908597.1	hypothetical protein	-
KNN07_RS23990 (KNN07_23990)	24402..24731	+	330	WP_000542383.1	hypothetical protein	-
KNN07_RS23995 (KNN07_23995)	24724..25917	+	1194	WP_074470350.1	phage tail protein	-
KNN07_RS24000 (KNN07_24000)	25951..26679	-	729	WP_199613379.1	hypothetical protein	-
KNN07_RS25135	26716..26901	-	186	Protein_46	hypothetical protein	-
KNN07_RS24005 (KNN07_24005)	26958..27296	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
KNN07_RS24010 (KNN07_24010)	27367..27669	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KNN07_RS24015 (KNN07_24015)	27832..28623	+	792	WP_199613380.1	phage tail protein	-
KNN07_RS24020 (KNN07_24020)	28620..29387	+	768	WP_199613381.1	baseplate	-
KNN07_RS24025 (KNN07_24025)	29391..30371	+	981	WP_000046500.1	hypothetical protein	-
KNN07_RS24030 (KNN07_24030)	30368..31021	+	654	WP_000828873.1	hypothetical protein	-
KNN07_RS24035 (KNN07_24035)	31081..31986	+	906	WP_048666481.1	recombination-associated protein RdgC	-
KNN07_RS24040 (KNN07_24040)	32030..32650	+	621	WP_206769821.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86764	86764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T205470 WP_000162415.1 NZ_CP076307:c27669-27367 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T205470 NZ_CP101667:45112-45399 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT205470 WP_000806445.1 NZ_CP076307:c27296-26958 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT205470 NZ_CP101667:44864-45115 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3