Detailed information of TA system TA205465

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36332..36586
• Replicon: plasmid pS253-NDM5
• Accession: NZ_CP076306
• Organism: Escherichia coli strain Survcare253

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: KNN07_RS23490
• Protein ID: WP_001367749.1
• Coordinates: 36332..36481 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	36525..36586 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN07_RS23455 (31691)	31691..32106	-	416	Protein_39	IS1-like element IS1B family transposase	-
KNN07_RS23460 (32355)	32355..32756	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KNN07_RS23465 (32689)	32689..32946	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KNN07_RS23470 (33039)	33039..33692	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KNN07_RS23475 (34631)	34631..35488	-	858	WP_163419524.1	incFII family plasmid replication initiator RepA	-
KNN07_RS23480 (35481)	35481..35555	-	75	WP_001365705.1	RepA leader peptide Tap	-
KNN07_RS23485 (35791)	35791..36048	-	258	WP_000083833.1	replication regulatory protein RepA	-
KNN07_RS23490 (36332)	36332..36481	-	150	WP_001367749.1	Hok/Gef family protein	Toxin
- (36525)	36525..36586	+	62	NuclAT_0	-	Antitoxin
- (36525)	36525..36586	+	62	NuclAT_0	-	Antitoxin
- (36525)	36525..36586	+	62	NuclAT_0	-	Antitoxin
- (36525)	36525..36586	+	62	NuclAT_0	-	Antitoxin
KNN07_RS25085 (36725)	36725..37153	-	429	WP_029702151.1	hypothetical protein	-
KNN07_RS23500 (37366)	37366..37935	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
KNN07_RS23505 (38087)	38087..38713	-	627	WP_029702149.1	NYN domain-containing protein	-
KNN07_RS23515 (39804)	39804..40421	+	618	Protein_51	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / mph(A) / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / qepA4 / tet(B)	-	1..89595	89595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T205465 WP_001367749.1 NZ_CP076306:c36481-36332 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205465 NZ_CP101666:3607908-3608222 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGTATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT205465 NZ_CP076306:36525-36586 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin