Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3915712..3916511 Replicon chromosome
Accession NZ_CP076305
Organism Escherichia coli strain Survcare253

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KNN07_RS19065 Protein ID WP_000347251.1
Coordinates 3916047..3916511 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KNN07_RS19060 Protein ID WP_001307405.1
Coordinates 3915712..3916047 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN07_RS19045 (3911497) 3911497..3912267 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
KNN07_RS19050 (3912283) 3912283..3913617 - 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
KNN07_RS19055 (3913992) 3913992..3915563 + 1572 WP_001273761.1 galactarate dehydratase -
KNN07_RS19060 (3915712) 3915712..3916047 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KNN07_RS19065 (3916047) 3916047..3916511 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KNN07_RS19070 (3916566) 3916566..3917375 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KNN07_RS19075 (3917624) 3917624..3918904 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KNN07_RS19080 (3918927) 3918927..3919400 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KNN07_RS19085 (3919411) 3919411..3920190 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KNN07_RS19090 (3920180) 3920180..3921058 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KNN07_RS19095 (3921076) 3921076..3921510 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3904950..3916511 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T205462 WP_000347251.1 NZ_CP076305:3916047-3916511 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T205462 NZ_CP101666:3057266-3057451 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205462 WP_001307405.1 NZ_CP076305:3915712-3916047 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205462 NZ_CP101666:3057468-3057878 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References