Detailed information of TA system TA205442

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43478..43742
• Replicon: plasmid pS321-CMY-42
• Accession: NZ_CP076304
• Organism: Escherichia coli strain Survcare321

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: KNN06_RS25835
• Protein ID: WP_001387489.1
• Coordinates: 43478..43630 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43682..43742 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN06_RS25800 (38570)	38570..39103	-	534	WP_001221666.1	lipocalin family protein	-
KNN06_RS25805 (39197)	39197..40342	-	1146	WP_099004447.1	CMY-2 family class C beta-lactamase	-
KNN06_RS26175 (41547)	41547..41645	+	99	Protein_48	ethanolamine utilization protein EutE	-
KNN06_RS25815 (41717)	41717..41926	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KNN06_RS25820 (42318)	42318..42494	+	177	WP_001054897.1	hypothetical protein	-
KNN06_RS26250 (42559)	42559..42654	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
KNN06_RS25830 (43155)	43155..43406	+	252	WP_001291964.1	hypothetical protein	-
KNN06_RS25835 (43478)	43478..43630	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (43682)	43682..43742	+	61	NuclAT_0	-	Antitoxin
- (43682)	43682..43742	+	61	NuclAT_0	-	Antitoxin
- (43682)	43682..43742	+	61	NuclAT_0	-	Antitoxin
- (43682)	43682..43742	+	61	NuclAT_0	-	Antitoxin
KNN06_RS25840 (43951)	43951..44325	+	375	WP_223349261.1	hypothetical protein	-
KNN06_RS25845 (44478)	44478..45635	+	1158	Protein_55	IS1380-like element ISEcp1 family transposase	-
KNN06_RS25850 (45691)	45691..46388	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KNN06_RS25855 (46399)	46399..46707	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
KNN06_RS25860 (47149)	47149..47436	-	288	WP_000074855.1	conjugal transfer protein TraA	-
KNN06_RS26180 (47474)	47474..47632	-	159	Protein_59	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48543	48543
	-	inside	IScluster/Tn	blaCMY-42	-	39197..45966	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T205442 WP_001387489.1 NZ_CP076304:c43630-43478 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T205442 NZ_CP101656:2856389-2856976 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGTGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT205442 NZ_CP076304:43682-43742 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin