Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4381578..4382272 Replicon chromosome
Accession NZ_CP076301
Organism Escherichia coli strain Survcare321

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag KNN06_RS21045 Protein ID WP_001521903.1
Coordinates 4381874..4382272 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KNN06_RS21040 Protein ID WP_000554758.1
Coordinates 4381578..4381871 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN06_RS21015 (4376693) 4376693..4377442 + 750 WP_016233951.1 C40 family peptidase -
KNN06_RS21020 (4377499) 4377499..4377852 - 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
KNN06_RS21025 (4377931) 4377931..4379643 - 1713 Protein_4134 flagellar biosynthesis protein FlhA -
KNN06_RS21030 (4379615) 4379615..4380400 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
KNN06_RS21035 (4380471) 4380471..4381526 + 1056 WP_001226177.1 DNA polymerase IV -
KNN06_RS21040 (4381578) 4381578..4381871 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KNN06_RS21045 (4381874) 4381874..4382272 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KNN06_RS21050 (4382282) 4382282..4382734 + 453 WP_023144376.1 GNAT family N-acetyltransferase -
KNN06_RS21055 (4382979) 4382979..4383185 + 207 Protein_4140 RtcB family protein -
KNN06_RS21060 (4383181) 4383181..4383533 + 353 Protein_4141 peptide chain release factor H -
KNN06_RS21065 (4383590) 4383590..4385047 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
KNN06_RS21070 (4385308) 4385308..4385766 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4386362) 4386362..4386442 + 81 NuclAT_10 - -
- (4386362) 4386362..4386442 + 81 NuclAT_10 - -
- (4386362) 4386362..4386442 + 81 NuclAT_10 - -
- (4386362) 4386362..4386442 + 81 NuclAT_10 - -
KNN06_RS21075 (4385858) 4385858..4387102 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T205432 WP_001521903.1 NZ_CP076301:4381874-4382272 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T205432 NZ_CP101648:2422469-2422572 [Salmonella enterica subsp. enterica serovar Kentucky]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT205432 WP_000554758.1 NZ_CP076301:4381578-4381871 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT205432 NZ_CP101648:c2422574-2422431 [Salmonella enterica subsp. enterica serovar Kentucky]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References