Detailed information of TA system TA205426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3429728..3430330
• Replicon: chromosome
• Accession: NZ_CP076301
• Organism: Escherichia coli strain Survcare321

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KNN06_RS16545
• Protein ID: WP_000897305.1
• Coordinates: 3429728..3430039 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KNN06_RS16550
• Protein ID: WP_000356395.1
• Coordinates: 3430040..3430330 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN06_RS16525 (3424769)	3424769..3425206	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KNN06_RS16530 (3425251)	3425251..3426192	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KNN06_RS26010 (3426608)	3426608..3427495	+	888	Protein_3256	hypothetical protein	-
KNN06_RS26015 (3427508)	3427508..3428422	+	915	WP_077634137.1	transposase	-
KNN06_RS16540 (3428591)	3428591..3429499	-	909	WP_001306651.1	alpha/beta hydrolase	-
KNN06_RS16545 (3429728)	3429728..3430039	+	312	WP_000897305.1	hypothetical protein	Toxin
KNN06_RS16550 (3430040)	3430040..3430330	+	291	WP_000356395.1	NadS family protein	Antitoxin
KNN06_RS16555 (3430415)	3430415..3430627	+	213	WP_000197769.1	hypothetical protein	-
KNN06_RS16560 (3430689)	3430689..3430967	+	279	WP_001306650.1	hypothetical protein	-
KNN06_RS16565 (3431386)	3431386..3431604	+	219	WP_001295676.1	CopG family transcriptional regulator	-
KNN06_RS16570 (3431823)	3431823..3432065	+	243	WP_021523315.1	hypothetical protein	-
KNN06_RS16575 (3432247)	3432247..3433176	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KNN06_RS16580 (3433173)	3433173..3433808	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KNN06_RS16585 (3433805)	3433805..3434707	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205426 WP_000897305.1 NZ_CP076301:3429728-3430039 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205426 NZ_CP101648:c154311-154000 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT205426 WP_000356395.1 NZ_CP076301:3430040-3430330 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205426 NZ_CP101648:c153999-153709 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTAAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGCTCACCGACAGGGCCAGCCAAAGCATTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7