Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prlF-yhaV (relBE)/YhaV-PrlF |
| Location | 2612090..2612889 | Replicon | chromosome |
| Accession | NZ_CP076301 | ||
| Organism | Escherichia coli strain Survcare321 | ||
Toxin (Protein)
| Gene name | yhaV | Uniprot ID | D3GWU5 |
| Locus tag | KNN06_RS12645 | Protein ID | WP_000347272.1 |
| Coordinates | 2612425..2612889 (+) | Length | 155 a.a. |
Antitoxin (Protein)
| Gene name | prlF | Uniprot ID | S1EB98 |
| Locus tag | KNN06_RS12640 | Protein ID | WP_001307405.1 |
| Coordinates | 2612090..2612425 (+) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KNN06_RS12625 (2607875) | 2607875..2608645 | - | 771 | WP_001058227.1 | 2-dehydro-3-deoxyglucarate aldolase | - |
| KNN06_RS12630 (2608661) | 2608661..2609995 | - | 1335 | WP_199613731.1 | galactarate/glucarate/glycerate transporter GarP | - |
| KNN06_RS12635 (2610370) | 2610370..2611941 | + | 1572 | WP_001273738.1 | galactarate dehydratase | - |
| KNN06_RS12640 (2612090) | 2612090..2612425 | + | 336 | WP_001307405.1 | type II toxin-antitoxin system antitoxin PrlF | Antitoxin |
| KNN06_RS12645 (2612425) | 2612425..2612889 | + | 465 | WP_000347272.1 | type II toxin-antitoxin system ribonuclease toxin YhaV | Toxin |
| KNN06_RS12650 (2612944) | 2612944..2613753 | - | 810 | WP_000072171.1 | aga operon transcriptional regulator AgaR | - |
| KNN06_RS12655 (2614002) | 2614002..2615282 | + | 1281 | WP_001521382.1 | tagatose-bisphosphate aldolase subunit KbaZ | - |
| KNN06_RS12660 (2615305) | 2615305..2615778 | + | 474 | WP_001295547.1 | PTS N-acetylgalactosamine transporter subunit IIB | - |
| KNN06_RS12665 (2615789) | 2615789..2616568 | + | 780 | WP_000406214.1 | PTS N-acetylgalactosamine transporter subunit IIC | - |
| KNN06_RS12670 (2616558) | 2616558..2617436 | + | 879 | WP_001298314.1 | PTS N-acetylgalactosamine transporter subunit IID | - |
| KNN06_RS12675 (2617454) | 2617454..2617888 | + | 435 | WP_000948824.1 | PTS galactosamine/N-acetylgalactosamine transporter subunit IIA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 2602565..2612889 | 10324 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 155 a.a. Molecular weight: 17832.26 Da Isoelectric Point: 9.6924
>T205425 WP_000347272.1 NZ_CP076301:2612425-2612889 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH
Download Length: 465 bp
>T205425 NZ_CP101647:c4647243-4646752 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGAACCATCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12358.94 Da Isoelectric Point: 4.8616
>AT205425 WP_001307405.1 NZ_CP076301:2612090-2612425 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
Download Length: 336 bp
>AT205425 NZ_CP101647:c4647533-4647240 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA
ATGTCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCACAGGCTTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829KUD2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVC7 |