Detailed information of TA system TA205424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 2504809..2505502
• Replicon: chromosome
• Accession: NZ_CP076301
• Organism: Escherichia coli strain Survcare321

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: KNN06_RS12120
• Protein ID: WP_000415584.1
• Coordinates: 2505206..2505502 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: KNN06_RS12115
• Protein ID: WP_000650107.1
• Coordinates: 2504809..2505204 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN06_RS12105 (2500673)	2500673..2502931	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
KNN06_RS12110 (2503069)	2503069..2504676	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
KNN06_RS12115 (2504809)	2504809..2505204	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
KNN06_RS12120 (2505206)	2505206..2505502	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
KNN06_RS12125 (2505707)	2505707..2506189	-	483	WP_000183505.1	GyrI-like domain-containing protein	-
KNN06_RS12130 (2506242)	2506242..2506634	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
KNN06_RS12135 (2506786)	2506786..2507445	+	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
KNN06_RS12140 (2507442)	2507442..2508791	+	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
KNN06_RS12145 (2508837)	2508837..2509169	-	333	WP_023149630.1	DUF2645 family protein	-
KNN06_RS12150 (2509488)	2509488..2510069	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
KNN06_RS12155 (2510100)	2510100..2510414	+	315	WP_000958598.1	putative quinol monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T205424 WP_000415584.1 NZ_CP076301:c2505502-2505206 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T205424 NZ_CP101647:4508793-4509209 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCTGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT205424 WP_000650107.1 NZ_CP076301:c2505204-2504809 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT205424 NZ_CP101647:4508566-4508796 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65