Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2027456..2028183 Replicon chromosome
Accession NZ_CP076301
Organism Escherichia coli strain Survcare321

Toxin (Protein)


Gene name higB Uniprot ID A0A829L2T9
Locus tag KNN06_RS09875 Protein ID WP_001521139.1
Coordinates 2027872..2028183 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KNN06_RS09870 Protein ID WP_000126294.1
Coordinates 2027456..2027875 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN06_RS09850 (2022541) 2022541..2023068 - 528 WP_001078777.1 electron transport protein HydN -
KNN06_RS09855 (2023217) 2023217..2024227 - 1011 WP_015912547.1 DNA-binding transcriptional regulator AscG -
KNN06_RS09860 (2024487) 2024487..2025944 + 1458 WP_020234027.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
KNN06_RS09865 (2025953) 2025953..2027377 + 1425 WP_020234028.1 6-phospho-beta-glucosidase AscB -
KNN06_RS09870 (2027456) 2027456..2027875 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
KNN06_RS09875 (2027872) 2027872..2028183 - 312 WP_001521139.1 type II toxin-antitoxin system HigB family toxin Toxin
KNN06_RS09880 (2028350) 2028350..2028820 - 471 WP_199613727.1 hydrogenase maturation peptidase HycI -
KNN06_RS09885 (2028813) 2028813..2029223 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
KNN06_RS09890 (2029220) 2029220..2029987 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
KNN06_RS09895 (2029987) 2029987..2030529 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
KNN06_RS09900 (2030539) 2030539..2032248 - 1710 WP_001521140.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12493.23 Da        Isoelectric Point: 9.4783

>T205420 WP_001521139.1 NZ_CP076301:c2028183-2027872 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T205420 NZ_CP101647:2422469-2422572 [Salmonella enterica subsp. enterica serovar Kentucky]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT205420 WP_000126294.1 NZ_CP076301:c2027875-2027456 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT205420 NZ_CP101647:c2422574-2422431 [Salmonella enterica subsp. enterica serovar Kentucky]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L2T9

Antitoxin

Source ID Structure

References