Detailed information of TA system TA205392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4104649..4105251
• Replicon: chromosome
• Accession: NZ_CP076285
• Organism: Escherichia coli strain 41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A7A12_RS19890
• Protein ID: WP_000897305.1
• Coordinates: 4104649..4104960 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7A12_RS19895
• Protein ID: WP_000356397.1
• Coordinates: 4104961..4105251 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A12_RS19860 (4099679)	4099679..4100464	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7A12_RS19865 (4100563)	4100563..4101162	+	600	WP_001295269.1	glucose-1-phosphatase	-
A7A12_RS19870 (4101156)	4101156..4102028	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A7A12_RS19875 (4102025)	4102025..4102462	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
A7A12_RS19880 (4102507)	4102507..4103448	+	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
A7A12_RS19885 (4103512)	4103512..4104420	-	909	WP_086563287.1	alpha/beta hydrolase	-
A7A12_RS19890 (4104649)	4104649..4104960	+	312	WP_000897305.1	hypothetical protein	Toxin
A7A12_RS19895 (4104961)	4104961..4105251	+	291	WP_000356397.1	NadS family protein	Antitoxin
A7A12_RS19900 (4105856)	4105856..4106074	+	219	WP_001297075.1	CopG family transcriptional regulator	-
A7A12_RS19905 (4106293)	4106293..4106535	+	243	WP_001086388.1	protein YiiF	-
A7A12_RS19910 (4106865)	4106865..4107794	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
A7A12_RS19915 (4107791)	4107791..4108426	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A7A12_RS19920 (4108423)	4108423..4109325	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T205392 WP_000897305.1 NZ_CP076285:4104649-4104960 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T205392 NZ_CP101615:1846918-1847169 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT205392 WP_000356397.1 NZ_CP076285:4104961-4105251 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT205392 NZ_CP101615:1847166-1847420 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7