Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 256718..257259 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag A7A12_RS01175 Protein ID WP_000615979.1
Coordinates 256718..256996 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A12_RS01180 Protein ID WP_000729704.1
Coordinates 256999..257259 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS01160 (254300) 254300..254878 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A12_RS01165 (255084) 255084..255851 + 768 WP_000333380.1 class II glutamine amidotransferase -
A7A12_RS01170 (255822) 255822..256562 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
A7A12_RS01175 (256718) 256718..256996 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A12_RS01180 (256999) 256999..257259 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A12_RS01185 (257445) 257445..258218 + 774 WP_000543899.1 C40 family peptidase -
A7A12_RS01190 (258275) 258275..258631 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A7A12_RS01195 (258624) 258624..258902 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A12_RS01200 (259007) 259007..260719 - 1713 Protein_232 flagellar biosynthesis protein FlhA -
A7A12_RS01205 (260691) 260691..261476 + 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T205368 WP_000615979.1 NZ_CP076285:c256996-256718 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205368 NZ_CP101595:c1166177-1165959 [Cronobacter dublinensis]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGCGACGCTGTCAGACAATTGATACGCTTGAGCGTGT
GATTGAAAAAAATAAATATGAATTGCCCGATAACGAACTGGCGGTATTTTATTCAGCAGCGGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAATCCCAACGTCCGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT205368 WP_000729704.1 NZ_CP076285:c257259-256999 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205368 NZ_CP101595:c1166578-1166204 [Cronobacter dublinensis]
ATGGACGAATACTCACCAAAACGGCATGATATGGCGCAGCTTAAATTTCTGTGTGAAAGCCTGTATCATGATTGCCTCGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACATCCGCCGTTAACCTGCAACTGAACGATCTGATTGAGC
ACATTGCCAGCTTCGCGCTTAATTATAAAATTAAGCATGAAGAGGATGCCGCTCTCATTGAACAATTGGATGAATATCTG
GACGATACCTTTATGCTTTTCAGCAATTACGGTATCAATGCGCAAGATTTGCAAAAATGGCGCAAGTCCGGAAACAGACT
TTTCCGGTGTTTCGTAAACGCCAGCCGGGAGAATCCTGTAAGCTTGTCTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References