Detailed information of TA system TA205361

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29056..29479
• Replicon: plasmid p4422IncF
• Accession: NZ_CP076283
• Organism: Escherichia coli strain 442/2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A15_RS23745
• Protein ID: WP_229484968.1
• Coordinates: 29056..29175 (-)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29263..29479 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A15_RS23710 (24099)	24099..24326	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
A7A15_RS23715 (24420)	24420..25106	-	687	WP_000332492.1	PAS domain-containing protein	-
A7A15_RS23720 (25296)	25296..25679	-	384	WP_000124980.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A15_RS23725 (26035)	26035..26637	+	603	WP_000243717.1	transglycosylase SLT domain-containing protein	-
A7A15_RS23730 (26932)	26932..27753	-	822	WP_001234477.1	DUF932 domain-containing protein	-
A7A15_RS23735 (27870)	27870..28157	-	288	WP_000107547.1	hypothetical protein	-
A7A15_RS23740 (28457)	28457..28763	+	307	Protein_37	hypothetical protein	-
A7A15_RS23745 (29056)	29056..29175	-	120	WP_229484968.1	Hok/Gef family protein	Toxin
A7A15_RS23750 (29123)	29123..29275	-	153	Protein_39	FlmC family protein	-
- (29263)	29263..29479	-	217	NuclAT_0	-	Antitoxin
- (29263)	29263..29479	-	217	NuclAT_0	-	Antitoxin
- (29263)	29263..29479	-	217	NuclAT_0	-	Antitoxin
- (29263)	29263..29479	-	217	NuclAT_0	-	Antitoxin
A7A15_RS23755 (29448)	29448..30210	-	763	Protein_40	plasmid SOS inhibition protein A	-
A7A15_RS23760 (30207)	30207..30614	-	408	WP_001261250.1	conjugation system SOS inhibitor PsiB	-
A7A15_RS23765 (30775)	30775..30954	+	180	WP_000618107.1	hypothetical protein	-
A7A15_RS23770 (31038)	31038..31430	-	393	WP_000272016.1	plasmid partitioning/stability family protein	-
A7A15_RS23775 (31434)	31434..32408	-	975	WP_021534625.1	plasmid segregation protein ParM	-
A7A15_RS23780 (32647)	32647..33021	-	375	WP_000752650.1	hypothetical protein	-
A7A15_RS23785 (33021)	33021..33653	-	633	WP_000312330.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..77995	77995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4455.25 Da
• Isoelectric Point: 8.2691

>T205361 WP_229484968.1 NZ_CP076283:c29175-29056 [Escherichia coli]
VCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESSGK

Nucleotide

• Length: 120 bp

>T205361 NZ_CP101591:c2844724-2844362 [Cronobacter dublinensis]
ATGACATGGACGGTGATTTTTACCCCTCGTTTTTATGAGTGGTATCAACAACAGCCCGAAGGATTGCAGGATCGTATCGC
CGCGCTGTTATGGAATTTACGCCACAGCGGCCCGTTGGTCGGGCGTCCGCTGGTGGATACGATAAAAGGCTCGCAGATCC
CCAATCTCAAAGAGCTGCGCGTTCAGTATGGCGGTTCGCCGTGGCGGGTGTTTTTTGCCTTCGACCCAACCCGTCAGGCT
GTGGTGCTGTGTGCAGGCAACAAGCGCGGTCACAAAGACTTTTATAAAACCCTAATCAGGCAGGCGGAGGAAGCGTTCTT
CCTGCATCTGCAAATCATGGAGAGTAAAAATGAAAACGCTTGA

Antitoxin

Nucleotide

• Length: 217 bp

>AT205361 NZ_CP076283:c29479-29263 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin