Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3454018..3454817 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag A7A15_RS16865 Protein ID WP_000347266.1
Coordinates 3454353..3454817 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag A7A15_RS16860 Protein ID WP_001307405.1
Coordinates 3454018..3454353 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS16845 (3449803) 3449803..3450573 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
A7A15_RS16850 (3450589) 3450589..3451923 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
A7A15_RS16855 (3452298) 3452298..3453869 + 1572 WP_001273741.1 galactarate dehydratase -
A7A15_RS16860 (3454018) 3454018..3454353 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
A7A15_RS16865 (3454353) 3454353..3454817 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
A7A15_RS16870 (3454872) 3454872..3455681 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
A7A15_RS16875 (3455930) 3455930..3457210 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
A7A15_RS16880 (3457233) 3457233..3457706 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
A7A15_RS16885 (3457717) 3457717..3458496 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
A7A15_RS16890 (3458486) 3458486..3459364 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
A7A15_RS16895 (3459382) 3459382..3459816 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3442083..3454817 12734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T205355 WP_000347266.1 NZ_CP076282:3454353-3454817 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T205355 NZ_CP101570:267-581 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT205355 WP_001307405.1 NZ_CP076282:3454018-3454353 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT205355 NZ_CP101570:1-279 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References