Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2197763..2198631 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag BQ2027_RS10115 Protein ID WP_010886136.1
Coordinates 2197763..2198140 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag BQ2027_RS10120 Protein ID WP_003409886.1
Coordinates 2198182..2198631 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS10065 (BQ2027_MB1981C) 2193552..2194004 - 453 WP_010950636.1 lipoprotein -
BQ2027_RS10070 (BQ2027_MB1982) 2194068..2194469 + 402 WP_003409869.1 hypothetical protein -
BQ2027_RS10075 2194462..2194644 - 183 WP_003409870.1 hypothetical protein -
BQ2027_RS10080 (BQ2027_MB1983C) 2194758..2195108 - 351 WP_003409871.1 hypothetical protein -
BQ2027_RS10085 (BQ2027_MB1984C) 2195119..2196021 - 903 WP_003409874.1 hypothetical protein -
BQ2027_RS10090 (BQ2027_MB1985C) 2196042..2196233 - 192 WP_003409876.1 hypothetical protein -
BQ2027_RS10095 (BQ2027_MB1986C) 2196234..2196530 - 297 WP_003409877.1 hypothetical protein -
BQ2027_RS10100 (BQ2027_MB1987) 2196770..2196985 + 216 WP_003409878.1 antitoxin -
BQ2027_RS10105 (BQ2027_MB1988) 2196982..2197293 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
BQ2027_RS10110 (BQ2027_MB1989C) 2197267..2197788 - 522 WP_010950637.1 hypothetical protein -
BQ2027_RS10115 (BQ2027_MB1990) 2197763..2198140 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
BQ2027_RS10120 (BQ2027_MB1991) 2198182..2198631 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
BQ2027_RS10125 (BQ2027_MB1992) 2198628..2199173 + 546 WP_003409891.1 SecB-like chaperone -
BQ2027_RS10130 (BQ2027_MB1993C) 2199062..2199676 - 615 WP_003901296.1 hypothetical protein -
BQ2027_RS10135 (BQ2027_MB1994C) 2199725..2200021 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
BQ2027_RS10140 (BQ2027_MB1995C) 2200018..2200269 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
BQ2027_RS10145 (BQ2027_MB1996) 2200256..2200750 + 495 WP_003899099.1 hypothetical protein -
BQ2027_RS10150 (BQ2027_MB1997C) 2200910..2201317 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
BQ2027_RS10155 (BQ2027_MB1997A) 2201321..2201593 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
BQ2027_RS10160 (BQ2027_MB1998C) 2201626..2202846 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T20534 WP_010886136.1 NC_002945:2197763-2198140 [Mycobacterium tuberculosis variant bovis AF2122/97]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T20534 NC_002945:2197763-2198140 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT20534 WP_003409886.1 NC_002945:2198182-2198631 [Mycobacterium tuberculosis variant bovis AF2122/97]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT20534 NC_002945:2198182-2198631 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References