Detailed information of TA system TA205325

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 128349..128602
• Replicon: plasmid pSA53IncF
• Accession: NZ_CP076280
• Organism: Escherichia coli strain SA53-3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A831FKU2
• Locus tag: A7A19_RS25460
• Protein ID: WP_064758536.1
• Coordinates: 128453..128602 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	128349..128405 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A19_RS25420 (123660)	123660..123872	+	213	WP_257122920.1	hypothetical protein	-
A7A19_RS25425 (124200)	124200..124304	+	105	Protein_117	transcriptional regulator	-
A7A19_RS25430 (124384)	124384..124809	+	426	WP_257115045.1	transposase	-
A7A19_RS25435 (124806)	124806..125156	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A7A19_RS25440 (125187)	125187..126800	+	1614	WP_257122655.1	IS66-like element ISEc23 family transposase	-
A7A19_RS25445 (126833)	126833..126949	+	117	Protein_121	transcriptional regulator	-
A7A19_RS25450 (126987)	126987..127577	+	591	WP_257122922.1	DUF2726 domain-containing protein	-
A7A19_RS25455 (127732)	127732..128197	+	466	Protein_123	hypothetical protein	-
- (128349)	128349..128405	-	57	NuclAT_0	-	Antitoxin
- (128349)	128349..128405	-	57	NuclAT_0	-	Antitoxin
- (128349)	128349..128405	-	57	NuclAT_0	-	Antitoxin
- (128349)	128349..128405	-	57	NuclAT_0	-	Antitoxin
A7A19_RS25460 (128453)	128453..128602	+	150	WP_064758536.1	Hok/Gef family protein	Toxin
A7A19_RS25465 (128886)	128886..129143	+	258	WP_063101962.1	replication regulatory protein RepA	-
A7A19_RS25470 (129160)	129160..129414	-	255	WP_236533440.1	replication protein RepA	-
A7A19_RS25475 (129405)	129405..129452	+	48	WP_236533442.1	hypothetical protein	-
A7A19_RS25480 (129445)	129445..130467	+	1023	WP_257122923.1	plasmid replication initiator RepA	-
A7A19_RS25485 (131819)	131819..132070	+	252	WP_001315600.1	HTH domain-containing protein	-
A7A19_RS25490 (132621)	132621..133181	-	561	WP_064181638.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..149690	149690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5556.69 Da
• Isoelectric Point: 8.7678

>T205325 WP_064758536.1 NZ_CP076280:128453-128602 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQITLAYEARK

Nucleotide

• Length: 150 bp

>T205325 NZ_CP101558:c4472-4182 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAACCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT205325 NZ_CP076280:c128405-128349 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A831FKU2

Antitoxin