Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4481609..4482211 Replicon chromosome
Accession NZ_CP076279
Organism Escherichia coli strain SA53-3

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag A7A19_RS22270 Protein ID WP_000897305.1
Coordinates 4481609..4481920 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag A7A19_RS22275 Protein ID WP_000356397.1
Coordinates 4481921..4482211 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A19_RS22240 (4476639) 4476639..4477424 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
A7A19_RS22245 (4477523) 4477523..4478122 + 600 WP_001295269.1 glucose-1-phosphatase -
A7A19_RS22250 (4478116) 4478116..4478988 + 873 WP_000920762.1 virulence factor BrkB family protein -
A7A19_RS22255 (4478985) 4478985..4479422 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
A7A19_RS22260 (4479467) 4479467..4480408 + 942 WP_001343389.1 fatty acid biosynthesis protein FabY -
A7A19_RS22265 (4480472) 4480472..4481380 - 909 WP_001162704.1 alpha/beta hydrolase -
A7A19_RS22270 (4481609) 4481609..4481920 + 312 WP_000897305.1 hypothetical protein Toxin
A7A19_RS22275 (4481921) 4481921..4482211 + 291 WP_000356397.1 NadS family protein Antitoxin
A7A19_RS22280 (4482797) 4482797..4483015 + 219 WP_001295676.1 CopG family transcriptional regulator -
A7A19_RS22285 (4483234) 4483234..4483476 + 243 WP_001087409.1 protein YiiF -
A7A19_RS22290 (4483805) 4483805..4484734 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
A7A19_RS22295 (4484731) 4484731..4485366 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
A7A19_RS22300 (4485363) 4485363..4486265 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T205323 WP_000897305.1 NZ_CP076279:4481609-4481920 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T205323 NZ_CP101556:c2648-2496 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT205323 WP_000356397.1 NZ_CP076279:4481921-4482211 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT205323 NZ_CP101556:2700-2760 [Klebsiella pneumoniae subsp. pneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References