Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 285672..286213 Replicon chromosome
Accession NZ_CP076279
Organism Escherichia coli strain SA53-3

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag A7A19_RS01270 Protein ID WP_000615982.1
Coordinates 285672..285950 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag A7A19_RS01275 Protein ID WP_000729704.1
Coordinates 285953..286213 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A19_RS01255 (283254) 283254..283832 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
A7A19_RS01260 (284038) 284038..284805 + 768 WP_000333380.1 class II glutamine amidotransferase -
A7A19_RS01265 (284776) 284776..285516 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
A7A19_RS01270 (285672) 285672..285950 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A7A19_RS01275 (285953) 285953..286213 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A7A19_RS01280 (286399) 286399..287172 + 774 WP_000543899.1 C40 family peptidase -
A7A19_RS01285 (287229) 287229..287585 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
A7A19_RS01290 (287578) 287578..287856 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
A7A19_RS01295 (287961) 287961..289673 - 1713 Protein_252 flagellar biosynthesis protein FlhA -
A7A19_RS01300 (289645) 289645..290430 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T205299 WP_000615982.1 NZ_CP076279:c285950-285672 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T205299 NZ_CP101546:836512-836922 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT205299 WP_000729704.1 NZ_CP076279:c286213-285953 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT205299 NZ_CP101546:836265-836531 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References