Detailed information of TA system TA205294

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45402..45666
• Replicon: plasmid pSA31IncI1
• Accession: NZ_CP076278
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: A7A21_RS24760
• Protein ID: WP_001387489.1
• Coordinates: 45402..45554 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	45604..45666 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS24725 (40519)	40519..41732	+	1214	WP_139371347.1	IS3 family transposase	-
A7A21_RS24730 (41774)	41774..43036	+	1263	Protein_45	IncI1-type conjugal transfer membrane protein TraY	-
A7A21_RS24735 (43112)	43112..43726	+	615	WP_000578648.1	plasmid IncI1-type surface exclusion protein ExcA	-
A7A21_RS24740 (43824)	43824..44033	+	210	WP_001140543.1	hemolysin expression modulator Hha	-
A7A21_RS24745 (44242)	44242..44418	+	177	WP_001054904.1	hypothetical protein	-
A7A21_RS24750 (44483)	44483..44779	-	297	WP_001275298.1	DinQ-like type I toxin DqlB	-
A7A21_RS24755 (45079)	45079..45330	+	252	WP_001291965.1	hypothetical protein	-
A7A21_RS24760 (45402)	45402..45554	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (45604)	45604..45666	+	63	NuclAT_0	-	Antitoxin
- (45604)	45604..45666	+	63	NuclAT_0	-	Antitoxin
- (45604)	45604..45666	+	63	NuclAT_0	-	Antitoxin
- (45604)	45604..45666	+	63	NuclAT_0	-	Antitoxin
- (45907)	45907..45962	+	56	NuclAT_1	-	-
- (45907)	45907..45962	+	56	NuclAT_1	-	-
- (45907)	45907..45962	+	56	NuclAT_1	-	-
- (45907)	45907..45962	+	56	NuclAT_1	-	-
A7A21_RS24765 (46156)	46156..47109	+	954	WP_021513958.1	hypothetical protein	-
A7A21_RS24770 (47327)	47327..48535	+	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
A7A21_RS24775 (48554)	48554..49624	+	1071	WP_257115099.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	basG	1..115220	115220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T205294 WP_001387489.1 NZ_CP076278:c45554-45402 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T205294 NZ_CP101544:c6347-6033 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT205294 NZ_CP076278:45604-45666 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin