Detailed information of TA system TA205289

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84315..84736
• Replicon: plasmid pSA31IncF
• Accession: NZ_CP076277
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: A7A21_RS24175
• Protein ID: WP_096937776.1
• Coordinates: 84315..84440 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	84538..84736 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS24135 (79354)	79354..79581	-	228	WP_257115011.1	conjugal transfer relaxosome protein TraY	-
A7A21_RS24140 (79719)	79719..80390	-	672	WP_040100314.1	conjugal transfer transcriptional regulator TraJ	-
A7A21_RS24145 (80585)	80585..80968	-	384	WP_040100321.1	conjugal transfer relaxosome DNA-binding protein TraM	-
A7A21_RS24150 (81292)	81292..81894	+	603	WP_000243701.1	transglycosylase SLT domain-containing protein	-
A7A21_RS24155 (82190)	82190..83011	-	822	WP_257115057.1	DUF932 domain-containing protein	-
A7A21_RS24160 (83130)	83130..83416	-	287	Protein_89	hypothetical protein	-
A7A21_RS24165 (83441)	83441..83647	-	207	WP_032219147.1	hypothetical protein	-
A7A21_RS24170 (83717)	83717..84014	+	298	Protein_91	hypothetical protein	-
A7A21_RS24175 (84315)	84315..84440	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
A7A21_RS24180 (84382)	84382..84531	-	150	Protein_93	DUF5431 family protein	-
- (84538)	84538..84736	-	199	NuclAT_0	-	Antitoxin
- (84538)	84538..84736	-	199	NuclAT_0	-	Antitoxin
- (84538)	84538..84736	-	199	NuclAT_0	-	Antitoxin
- (84538)	84538..84736	-	199	NuclAT_0	-	Antitoxin
A7A21_RS24185 (84705)	84705..85467	-	763	Protein_94	plasmid SOS inhibition protein A	-
A7A21_RS24190 (85464)	85464..85898	-	435	WP_096100127.1	conjugation system SOS inhibitor PsiB	-
A7A21_RS24195 (85953)	85953..87912	-	1960	Protein_96	ParB/RepB/Spo0J family partition protein	-
A7A21_RS24200 (87976)	87976..88209	-	234	WP_000005988.1	DUF905 family protein	-
A7A21_RS24205 (88272)	88272..88811	-	540	WP_032219151.1	single-stranded DNA-binding protein	-
A7A21_RS24210 (89112)	89112..89570	+	459	Protein_99	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..149410	149410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T205289 WP_096937776.1 NZ_CP076277:c84440-84315 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205289 NZ_CP101542:c4917084-4916800 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT205289 NZ_CP076277:c84736-84538 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin